The Enzyme Linked Immunosorbent Assay (ELISA)

The ELISA, or Enzyme-Linked ImmunoSorbent Assay, is an analytical biochemistry technique that uses antibodies to detect the presence of specific biomolecules (i.e. peptides, proteins, antigens and hormones) in a complex sample. These samples can be single proteins or complex mixtures like cellular lysates.

In this video, we will show you the basics of how to perform an ELISA in your classroom laboratory.

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[timothyow]

Background music is very upbeat considering someone has just tested positive for HIV.

[heshamyahya]

the background music was just ✨perfection✨

[heizeljoycecruz]

Hi! I am a Biology student. According to my research, ELISA Method can be used to detect Cyanobacteria on freshwater. May I know how am I going to detect the cyanobacteria in the freshwater? What procedure will I use/follow? Thank you so much! your answer will be a big help for us, future researchers.

[thejuryofwolves]

Super helpful video, and I liked the background music. It's upbeat and keeps me focused and entertained too.

[rizqirizaldo]

I will probably put the background music in the lab as well, that was a banger

[christinea]

THANK YOU SOOOO MUCH FOR THE VIDEO! I can finally understand this journal article im reading :")

[frankreiserm.s.]

Nice, quick video. I am surprised that you can use the same transfer pipet for every well for most steps.
Frank

[Vishwa]

There are very few professional s in India to do Elisa in a large scale. Diagnostic laboratory should take brave step for Covid19

[alexgunawan]

Anyway we can learn this and do a easy test like using strip?

[ahaaaihittop]

Is ELISA and Rapid antibody test HIV 1 AND 2 both Reliable?

[MrTurtleOfDoom]

New to ELISA! I have a quick question: is the color change because the detection substrate has interacted with the "sandwiched" antigen? I'm guessing no color changed means we don't have the antigen we are looking for?🤔

[josettenaadromo]

please, when do you add your sample if you are to purchase this instrument. Can it be used for detecting viruses?

[thuhanguyen]

Please why need used to the stop solution? Thanks

[sandygamertube]

l cant know where will we use the primary antibody and what is the reagent and its function

[sarahsuper]

Apparently, the ELISA Method can be used to detect BPA on water. May I know how am I going to detect the Bisphenol A in the water? What procedure will I use/follow? Thank you so much! your answer will be a big help for me!

[dominiqueskinner]

The background music is louder than his voice-over makes it hard to hear him talk.

[brandonwoolsey]

does the absorbance parallel the concentration? Could they be ran through a spectrophotometer to determine antibody concentration?

[sivaramreddy]

What if steps (provedure) are not followed ??

[khurramshahzad]

Hi! This test is used for anti hcv detection??