Elisa Test | Direct Indirect & Sandwich 🩸

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Elisa Test | Direct Indirect & Sandwich 🩸

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▬▬▬▬▬▬▬▬▬▬ Contents of this video ▬▬▬▬▬▬▬▬▬▬

00:00:00 What Is ELISA?
00:00:40 Explanation Of Mechanism Through An Example
00:09:57 Addition Of Substrate
00:11:55 Advantages Of Direct ELISA Test
00:13:58 Issues And Modifications Of ELISA
00:19:50 What Is Epitope?
00:22:01 Indirect Elisa Test And Its Mechanism
00:26:50 Performance Of Direct And Indirect Elisa Test
00:33:48 Effect Of Adding More Enzymes
00:34:40 When To Use Direct Or Indirect Test
00:39:44 Purpose Of Using These Two Tests Together
00:42:32 Sandwich Modification
00:49:37 Difference Between Direct And Direct Sandwich Elisa
00:51:03 Indirect Sandwich Test
00:54:27 Rapid Summary
00:58:07 Question
01:01:25 Radioimmuno Assay And Its Comparison With ELISA

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The ELISA test is simple and straightforward. You’ll probably need to sign a consent form, and your doctor should explain the reason for doing the test.

The ELISA test involves taking a sample of your blood. First, a healthcare provider will cleanse your arm with an antiseptic. Then, a tourniquet, or band, will be applied around your arm to create pressure and cause your veins to swell with blood. Next, a needle will be placed in one of your veins to draw a small sample of blood. When enough blood has been collected, the needle will be removed and a small bandage will be placed on your arm where the needle was. You’ll be asked to maintain pressure at the site where the needle was inserted for a few minutes to reduce blood flow.

This procedure should be relatively painless, but your arm may throb a little after it’s done.

The blood sample will be sent to a laboratory for analysis. In the lab, a technician will add the sample to a petri dish containing the specific antigen related to the condition for which you are being tested. If your blood contains antibodies to the antigen, the two will bind together. The technician will check this by adding an enzyme to the petri dish and observing how your blood and the antigen react.

You may have the condition if the contents of the dish change color. How much change the enzyme causes allows the technician to determine the presence and amount of antibody
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[souptikkabiraj]

Exceptional video...ELISA was never so easy until Dr. Najeeb came

[junaidchoudhury]

I wish i had a teacher like u .. hardly i came across such a teacher who teaches with so much simplicity like u ...Great work sir..trillions of thanks will also be microscopic 😁😁 for u... still infinite, infinite thanks to you .

[Dvzy]

The topics which u don't understand anywhere in the world.. This is the r a blessing for medical students sir...after watching this I can teach ELISA😁

Dr. you're not only a blessing for MBBS students but for other health professionals as well. I am in the 4th semester of my pharmacy school and literally your lectures help build up my concepts a lot. I can't imagine passing with good marks without listening to your lectures. The lectures are fun and I never get fed up with long lectures of yours. JazakAllah, thanks a lot. May Allah bless you, Ameen !!! :) You're an inspiration, keep up the gooD work :D

[ananya]

No video on youtube can beat how Najeeb sir has explained this concept thank you sir

[mohamedmagdy]

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[DocRj]

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[amosrechard]

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[sivakumardanisan]

Magnificent way of teaching which cleared the doubt that I have had for 2 thanks sir. I extremely loved it

[anusreebr]

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[RiyaSharma-guuq]

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I have my exam tomorrow and this video will help me score really Good.
Thank you

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[hanaineu]

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[julietesther]

Wonderful teacher....A techer should be like u...

[naveedkhankakar]

Salute to you sir. Believe me I haven't watched a movie with that amount of concentration as I do watch his lectures.
Thank you sir.

[ellaannechii]

You made me understand ELISA in just an hour. Keep the passion burning! You are a very good teacher. <3

[devashish]

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He is the saviour of medical students like me.

Love from India❤️

[UmarUsmanKhalil-uiwq]

I found this profoundly helpful. Thank you sir!

[mfatwaramadhan]

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