ELISA (Enzyme-linked Immunosorbent Assay)

Hey Friends,

ELISA, short for 'Enzyme-linked Immunosorbent Assay', is a powerful technique to detect substrates (e.g. an antigen). There are multiple subtypes and variants of ELISA. Here, the most common Sandwich ELISA is explained.

More about the DIRECT ELISA:

More about the INDIRECT ELISA:

An article about the Enzyme-linked Immunosorbent Assay:

Cheers
Henrik

Time Code:
0:00 What is an ELISA?
2:00 Sandwich ELISA example

[henrikslab]

From now on you can become a member of this channel! You will have access to some cool emojis like this:.
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[wodecka]

dude, you're saving my life for tomorrow's biochem module

[StevenMP]

The one video on ELISA that actually helped me understand. Thanks man!

[MAZINO]

Today is my biochem University. Thank you

[sarcicek]

I've discovered your channel yet. It is amazing for molecular biology students. 🙏🎉

[nicolevillalon]

Beautifully clear and concise explanation. Thank you!!!

[yelxi]

Thank You, Sir. You made it so easy to understand.

[TechMindSensei]

Precise concise and accurate thanks alot

[priya]

thia channel deserves more than the views it already has ❤🎉

[mayaskaaa]

so easily explained. thank you so much ❤

[mahmad]

Thankyou very much. You made it so simple

[gilian]

Beer's law -- applied to proteins; that's neat. But it looks like the assessment to determine the quantity of protein in a sample is strongly dependent on historical data; where you can compare similar samples to each other. ... ... or just read the data sheet provided by the supplier of the ELISA. After doing some reading on this... it looks like most ELISA samples are actually created by specialized laboratories that hire a lot of Peptide Chemists. Surprisingly... Peptide Chemists are paid around $45, 000-75, 000 per year (well; for ONE datapoint, but that ONE datapoint was requiring that the applicant had a PhD). I am a bit surprised at how saturated the Chemistry job market really is at the moment; that seems like low pay for pretty highly skilled work.

[guerroujadam]

man u made it so easy to understand even for a Frenchman ty so much ur video is easier to understand than my lecon xd

[sunberry]

Thanks, it's actually very useful.

[yeukaiwadzanaingwazane]

Thank you very much.. your videos are helpful

[pacokolb]

So whats the diffrence between western blot and elisa indirect variation?

[Raphaelpoh]

Which type of ELISA is suitable for very small viruses such as mouse parvovirus?

[sanjaykumar-ewmb]

❤ thanks for the nice video dear sir ❤

[ornulusoundeffects]

Is this the same thing what we do in SDS page?