ELISA- Enzyme Linked Immunosorbent Assay

Immunochemical reaction
sensitive, specific, easy in automation

Enzymes
Substrates

Types

Direct
Indirect
Sandwich
Competitive

Uses
Detection of Peptides, proteins, hormones, bacterial antigen, viral antigen
Detection of antibodies

[himanshuambilkar]

Elisa-
Enzyme linked immuno sorbent assay.
Detection of
Protein
Peptide
Hormone
Bacterial
Viral...

Ria radiation hazard
Shorten life...

Elisa no radiation
Longer shelf life
Easy automation...

Immunochemical method b/w Ag and Ab in which one of the immuno reagents is immobilized through solid phase support pvc or polystyrene through passive

Enzyme-
Horseradish peroxidase
All
B glucosidase
Glu oxidase
Ach E....

Linked
Glutaldehyde
...

Assay-
Direct Ag
Indirect Ab
Sandwich Ag
Competitive small Ag...

Direct-
Substrate TMB tetra methyl benzidine
Amino salicylic acid
OPD Ortho phenyldiamine
Azino di diammonium salt...


Indirect-
Hiv...

Sandwich -
Avidin biotin chemistry
TSH..

Competitive


Thank you sir

[meghanac]

if i started gain interest in biochemistry then that's because of your explanation sir, i reall love your explanation.

[VR-vc]

The best way of explanation of ELISA ever.

JUST BY SIMPLE RESOURCES.
Traditional chalk and duster.

[rhfitness]

Your class more more better than my college class.... Live sir 🤎🤎🤎

[shuchitabajpai]

I've seen so many videos related to elisa but none of them made me understand what it is actually about thank you so much sir this video helped me a lottt your teaching way was just perfect it was neither tough nor easy, appreciable ❤❤

[rajushaikh]

Thank you sir for your ELISA class .I am a student of dmlt in State medical faculty of west bengal

[muhammadtaimurkhan]

You always prepare your lectures deligently i have covered many of lectures you have explained it eloquently no confusion at all.
Islamabad, Pakistan

[meghanac]

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[sagnikbanik]

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[qwertypoiuytrewq]

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Thanku soo much 😌

[ABULHASAN-jwpb]

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[nilimashirke]

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[rittickadhikary]

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[RameshKumar-otzi]

Simple and superb sir 🙏
Thankyou so much 👍

[Bakeyblue]

Best of the best explanation, salute to you Sir 🙏

[kuldeepchouhan]

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[anchaltyagi]

BEST EXPLANATION.
THANK YOU SO MUCH SIR.

[fahadpasha]

Love you so much sir 😊thank you for making such concept easy

[manju-gstt]

Thanku so much sir
Topic itna easily samjha aagya.

[kirankarna]

Thanks for the video sir and wating to see 100k