What is a Western Blot?

0:00 Introduction
0:24 How do you determine the sex of a baby turtle?
1:30 What is a Western Blot?
2:43 How to interpret a Western Blot
3:10 How does a Western Blot work?
3:22 Electrophoresis (& What is a Coomassie Stain?)
4:57 Transfer
6:09 Blotting
7:19 Why is it called a "Western Blot?"
7:50 Western Blot = Immunoblot & Immunoassay
8:02 Results from research study using a Western Blot to determine the sex of baby turtles

The paper about determining the sex of baby turtles is:

Photo, Video and Music Credits:

Video of baby leatherback turtles leaving nest by Tyler Karaszewski CC-BY 2.0
Photo of snorkeling scientist with turtle by NOAA – Public Domain
Photo of scientist preparing mounting on turtle by Adam Li, NOAA/NMFS/SWFSC CC-BY 2.0
Example Western Blot CC-BY 4.0 from "Angiostrongylus cantonensis cathepsin B-like protease (Ac-cathB-1) is involved in host gut penetration". Parasite 22: 37. DOI:10.1051/parasite/2015037
Image of gel electrophoresis of DNA gel on UV box by TransControl CC-BY SA 3.0
Image of DNA gel adapted from R. Patra, et al., Multiple Infection and Microdiversity among Helicobacter pylori Isolates in a Single Host in India. PLOS ONE 7, e43370 (2012).
Mouse, Rabbit, and Goat cartoons by DataBase Center for Life Science (DBCLS) CC-BY 4.0
Photo of Gel Doc by Wiki210397 CC-BY SA 4.0
Photo of Edwin Southern by Gitschier J (2013) Problem Solved: An Interview with Sir Edwin Southern. PLoS Genet 9(3): e1003344. CC-BY 4.0

Hammock Fight music by Kevin MacLeod is licensed under a Creative Commons Attribution 4.0 license.

Photos and video of SDS-PAGE and Western Blot steps by Allie McClure and Berta Canal, used with permission.

Many thanks to Shannon Michele for letting me borrow her tortoise.

This video is copyright Jayme Dyer 2021, published under the Creative Commons CC-BY SA 4.0 license.

[sara]

you are a great teacher and your way of explaining things is unique. thank you

[science-learner]

These are incredible videos! I love how you explain the techniques using case studies and the why and how behind these also. Would love to know if you teach courses!

[gisellegastellou]

This was a great video, thank you so much!

[thinkerscientist]

A great video, really well explained how a Western blot works! I wish I had seen it something like this quite a few years ago when I was doing my first blots. Let´s just say they didn´t turn out very well ;)

[alejolab]

Another cool tool for the list 😁
But I wonder... why not having fluorescent markers on the primary antibodies directly?

[lilafromyoutube]

hahaha!! good thing you didn't ask siri

[terranovarubacha]

Now I need to know more about turtle conservation methods <dives down what is sure to be a rabbit hole>