Western Blotting | Beginner Data Interpretation Tutorial & Step-by-Step Protocol Explained

This is what you need to know about Western Blotting. The crash course.

Western Blotting is a method for analysing proteins, used by biologists.

I introduce western blotting figures, and an example experiment to understand how to read bands.

Pop any questions you have in the comment section and I'll do my best to answer them. BUT- disclaimer- I'm just a grad student with a few years of hands-on experience & I'm not an expert.

Want to talk science with me?

On this channel I talk about life as a PhD student in Scotland and other things. Check them out!

Pictures taken by Carmen Espejo Serrano & video by Ana Boavida. Diagrams made using Biorender.

#Biology #WesternBlotting #BiologyMajor #Immunology #CrashCourse #Cancer #CancerBiology

[sophiaharley]

You have explained this better in 10 minutes than an entire module on Western Blotting at university has!! Thank you!

[joaopauloferreirarodrigues]

The loading control tells me that there is possibly no band with 0 treatment because too little sample was loaded. This blotting needs to be repeated. Great video. Where GAPDH is weakest is precisely where we don't see band or expression is decreased. Great lecture!

[judybohnert]

This is such an informative video. I just recently started doing Western Blotting and was confused about reading the blot itself, but you explained several good points that have really helped me out! Thank you so very much for doing this video!

[Paprika]

Best explanation on the web, thank you! I've been searching far and wide for this.

[livinghope]

You are simply BEAUTIFUL with BEAUTIFUL WORK... I Love you🤣, so I subs + liked ur videos... Thank you, Emma!! From Master's student.

[casualcasual]

Thanks so much for the clear explanations! Hope to see more videos on protocols and data analyses, it helped me a lot :)

[manuelpv]

As a non native English speaker, I take note of "You can see the levels are not entirely consistent, this is a genuine Western Blot I did and it's not perfect. I believe this sentence will be of help to me.

Jokes aside, very insightful video, I enjoyed it a lot.

[razasyed]

yo big dawg ur actually the man helping people out, we Native Americans truly appreciate it

[deanortiz]

Incredible video! Thanks for sharing this information!

[williamarchacki]

Thanks so much! Comprehensive + concise (and it's interesting that you show why this technique matters with the example from your cancer research!)

[Lozlaloser]

such an incredibly helpful video can't even begin to describe, thank you

[thefenerbahcesk]

Thanks so much for making these videos, Emma. It's nice to have a secondary resource to review these methods besides the people in your lab - especially if the people in your lab get annoyed every time you ask questions.

[virgirma_PhD_scholar]

Perfect explaining Dr !Thanks you very much!

[veterinarytechnology]

Thank you prof. for your clear explanations

[s.myousufuddin]

Thank You For this awesome tutorial! This cleared all my confusions.

[nbent]

Thanks for the video! It was really helpful and straight to the point!

[williamsutherling]

Excellent, concise explanation. Thanks.

[calebboyer]

this was so helpful, thank you!! other videos were super confusing but this made so much sense :)

[justinpressley]

This was extremely helpful. Thank you so much!

[wycliffenyandika]

thanks for the explanation, hope you have more like this