Western Blot - Theory and method

Hello everybody and welcome to my western blot tutorial video.

In this video I will outline the methodology behind the western blot and how to perform it.

As usual if you leave a like and a comment if you have any further questions in regards to the video, thank you!

[Vekey]

This video was everything I wanted! I have a vague idea of everything in here and just needed a refresher on the actual practical use of everything. Thank you!

[SerenSanguis]

Very nice! The closest thing we have to lab practice in the pandemic!

[vishk]

Thank you for inviting me to your game! I played and learnt a lot!

[hirosewill]

Nice protocol and instruction. Thank you for the video

[JacquelineNettnin]

Thank you! This was a good explanation!

[AmandeepSingh-vcwj]

POV: You searched this video because you placed the membrane on the wrong side and lost your sample, so you're repeating the experiment again...

[Lina-]

Thank you! Great video, it helps me a lot!

[tle]

Good video but please
14:51 dont hold the pipette upside down with a used tip on it... ;)

[prembobby]

Everything clearly explained to more useful to practice this Western Blotting
Thanking you Sir/Mam

[Biomeducated]

Great tutorial! But,
13:58 you didn't depict the amplification tree (usually the secondary is polyclonal), so there's multiple secondaries binding to the primary to 'blow up' your signal from the HRP reaction.

Besides that: *great channel* here, man! Why did you stop though :(

Just as I'm discovering all these helpful channels... I even made a Top 10 channel overview on my own channel, and I'm planning on making a second one with 'smaller channels' like this one. All good stuff for Biomedical/Life Science students!

[amymay]

Thanks, great video! How does the metal stirrer help the process? I usually leave the tank without it being stirred the whole time, would love to know how stirring it helps as I may try that! Thanks so much in advance.

[valentinascatizzi]

thank you, brilliant and accurate explanation

[BalajiHariPhD]

I'm interested to do this western blot techniques

[ماجدفرج-خط]

Nice video, how long for blot
ting at 115 volt?

[汪华志]

i have a question : how tween inhibits detection ? what is the reaction mechanism

[AwaisAli-xlpu]

Hello Sir, I have facing repeated problem with my bands in WB, can you help me? Same problem persist despite of all repeated correction. Trouble shoot i am unable to identify

[ChristyLuvz]

Can the primary and secondary antibodies be diluted in the 5% non-fat milk and then each incubated with the membrane? Rather than doing the blocking step as its on whole step?

[nevinvolminck]

my mebrane disloved immedietly in the methanol :(

[Airisweetheart]

Dont they make you put your hair up in a lab?

[Buongona]

so blotting is blotting? I guess I really need to dig deeper...