Western Blotting

This video demonstrates SDS-PAGE separation of proteins using the Bio-Rad Comparative Proteomics Kit II: Western Blot Module. Assembly of the blotting sandwich and electroblotting are shown along with the steps for protein detection using a colorimetric assay.

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[nikkibryceroque]

I only watched this because of my assignment and gosh I've never been so overwhelmed by such laborious procedures!

[michelebos]

Thank you for this video, it really helpes me to understand correctly what i'm doing, when and how. Last week was the first time i had to do this proces but it was all unclear to me. Now I know what to do . Thank you so much !

[yodaydyxz]

Very informative
Thank you so much for taking time and explain each step.

[Aparna]

10ml of primary Ab and 10ml of substrate volumes were SO different, this does not feel right. I've never done a western blot, can someone explain which one was wrong? It kind of looks like 100ml of substrate

[muhammadyudadwitamaagustia]

when he was discarded the wash buffer or antibody, it gave me anxiety

[lamhirhcn]

Add 10 mL of primary antibody and pour off it...reminding me of the time when I sealed 2 mL of the primary antibody along with the membrane for binding and then recycled the used primary antibody...

[rfvcmtd]

WATCH IN X1.25 SPEED just to save you some time

[laurentiaadinda]

"pour off the primary and secondary antibody"

me watching this: o m g, i sealed and reused for many times bcs expensive😂😭

[dr.farzanarashidonny]

What kind of roller are you using here?
Where can I buy this?

[adronung]

What is the composition of blotting buffer, blocking buffer, wash buffer?

[starrynight]

Thank You so much for your wonderful video!

[menglinzhao]

Hi, I want to know what kinds of Substrate you used? The blue color you can see through the naked eyes or have you used a tool to analyze?

[asma-a]

all respect for this valuable work, thank you 🎩🌸

[stephenchetwynd]

Im trying to buy one of the plastic cases that you build the transfer casette shown in the above video but cant seem to find anyone selling.. what are they actually called?

[الإسلامسبيلي]

Helpful video, thank you. A piece of question, though; I expected chemiluminescent detection or use of detection equipment in general to visualize the bands after the secondary antibody but the video shows direct color development through substrate addition, would you clarify?

[tutoradvance]

I see this for sale on the wards scientific, but says the kit does not include fish samples, I can't find where I am suppose to buy the fish samples from?

[pgrwm]

Do you use nitril gloves? Are they resistant in contact with methanol in the Buffer?

[Birs_]

Thanks for this video. It's been a while since I did my last WB, so thanks for a short refreshing. But don't you think the 10 mL of substrate solution were significantly more than the 10 mL of both antibody solutions? ^_^

[trilochans]

How much duration of time take to diagnose western blot test for hiv in lab???(not about window period)

[marcussmith]

I have a question that might be answered here. I'm trying to get by cheaply and was wondering if I could run a western blot gel in a DGGE electrophoresis chamber ?