Illumina sequencing | DNA sequencing by synthesis

Illumina sequencing by synthesis - illumina sequencing process is explained in this video lecture. Illumina is one type of second generation DNA sequencing technique to get the sequence if target DNA easily and fast.
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The “sequencing-by using-synthesis” science now utilized by Illumina used to be at first developed by using Shankar Balasubramanian and David Klenerman at the college of Cambridge. They headquartered the company Solexa in 1998 to commercialize their sequencing procedure. Illumina went on to purchase Solexa in 2007 and has built upon, and speedily accelerated the long-established technology.
Illumina sequencers are presently probably the most generally used sequencing platform within the subsequent-new release sequencing (NGS) field. Illumina uses drift-cellphone floor for clustering DNA by way of ‘bridging amplification’, which generates clusters with hundreds of thousands of identical, single-stranded (ss), floor-hooked up DNA molecules After primer annealing, fluorescently labeled dATP, dGTP, dCTP and dTTP are introduced to the three′ end of the primer in line with the complementary base of the template strand. The fluorescently labeled nucleotides are chemically blanketed at the 3′ hydroxyl staff, which prevents the addition of greater than a single nucleotide per cycle. The digital camera then takes a snapshot of the go with the flow cellphone to realize the fluorescence from the final incorporated nucleotide of each cluster. The 3′ hydroxyl protection group as well as the fluorophore is enzymatically cleaved to proceed to the following cycle of the sequencing reaction. This stepwise addition of sequencing reactions is fascinating when sequencing homopolymer (repeating stretch of 1 form of nucleotide), which is often complicated for different sequencing platforms147. Moreover, the throughput of Illumina sequencers per sequencing run is 10–one hundred instances bigger than that of other sequencing platforms. Paired-end sequencing capabilities are additionally well headquartered, and these can make amends for the shorter learn length and present extended accuracy by studying the equal DNA template twice. The capabilities problem of Illumina sequencing technology is that the buildup of uncleaved fluorophores or protection agencies from each and every step can set off excessive noise and expand substitution error within the later sequencing cycles.

[Ametsi]

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[bishalgurung]

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[somnathbanerjee]

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[nowshinjahan]

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[UmerKhan-kdus]

thank u sir when i am unable to understand any topic i opened your videos and and i get solution of my problem there .you are very intelligent and way of teaching is mindblowing

[adekunlemaryam]

The way you explain these things, it makes it easy to understand

[BRENNANSTECHBITE]

Can you make a video on epigenetics, bisulfite conversion, methylation-specific PCR, and bisulfite sequencing?

Thanks!

[christinejackson]

Thank you for the excellent explanation of the bridge amplification method.

[cellbioinfo]

Thank You so much bro... You are so good all the way... Proud to be Bioinformatitian... Love You...

[bbeecckkyyss]

thank you for this video, it is the best explanation of Illumina sequencing we can get!!!

[sumitkumar-elkc]

Thank you so much for making concepts understandable.

[jamalinoor]

Excellent explanation, thank you shomu

[humairahr]

Thankyou Shomu this is the best explanation :)

[poonamvijaypuriya]

Thank u very much sir . U made this topic very easy to understand.

[shaswatadutta]

Sir is there any recommended book to read sequencing?
Thank you so much for the explanation

[farheenkhanam]

thank u much this vidio .it is really very helpful to me

[melanieferrace]

Thank you Shomu ! Excellent explanation, so helpful as always :D

[jacquelinelabovitz]

SHOMU! you are amazing! keep up the good work

[sonytambattilsonytambattil]

Sir can you upload a vedio regarding the relaxation of sequnece reads in bioinformatics?

[arnitheking]

Hi, can you explain to me why we need 2 reads when sequencing? Also, is barcoding necessary when analyzing self "made" (PCR) 16s rRNA gene fragments? or is it only useful when you get samples from libraries?