Illumina Sequencing Overview: Library Prep to Data Analysis | Webinar | Ambry Genetics

Scott Westenberger, Staff FAS with Illumina, will present an introduction to the Illumina Sequencing Workflow for Next Generation Sequencing. This presentation will cover the library preparation construct needed for sequencing on Illumina platforms, and the biochemical processes of cluster generation and sequencing by synthesis that are automatically performed by Illumina sequencing devices. This will provide a general overview of the process which applies to any assay across all different sequencing instruments, providing some comparison and contrast between different chemistry versions and instrument engineering developments.

Presented By:
Scott Westenberger, PhD | Presenter
Staff Clinical Field Applications Scientist, Illumina

Staff Field Applications Scientist with over 10 years in biotech industry supporting next generation sequencing and microarray devices, and over 20 years in molecular biology and genetics research. Specialist in Clinical Oncology, focused on supporting CLIA labs running NGS IVDs and Lab Developed Tests and Pharma and CROs engaged in translational research, clinical trials and companion diagnostics. Ph.D from UCLA in 2006 in Microbiology, Immunology and Molecular Genetics. Post-doc at The Scripps Research Institute in San Diego focused on parasite genomics and novel antimalarial drug screening.

Tara Namey, MS, LCGC | Moderator
Sr. Genomic Science Liaison, Ambry Genetics

Tara Namey joined Ambry Genetics in 2016 as an Oncology Genetic Specialist. Prior to joining Ambry, Tara was employed for 16 years at Lehigh Valley Health Network during which time she played an integral role in the development and growth of the Greg and Lorraine Harper Cancer Genetics Program. During her time there, Tara served as both Manager and Senior Genetic Counselor. Tara received her Bachelors of Science degree in Biology/Pre-Med from the University of Scranton. She earned her Masters of Science degree in Genetic Counseling from California State University, Northridge and is board-certified by the American Board of Genetic Counseling.

[nostalgia]

It’s so frustrating having to look up resources to learn something because the people who are suppose to teach/train you just put a picture on a slide and expect you to just know from a picture. This is the best explanation I could find also

[zeinabaltoufaili]

Best explanation of Sequencing by Synthesis that I could find! Thank you

[davidgangitano]

Excellent presentation and very clear about a technically sophisticated topic, Dr Westenberger!!!! Beste, Dr. G.

[Happy_Salchicha]

Thank you, finally a well-done explanation! Very helpful!

[igoruporov]

Very nice presentation! Clear and short. I'll recommend it to my students to learn most popular platform of NGS and excellent english. Thank you very much.

[yudhkaew]

It's very new and difficult for me. But after watching this video, I'm clearly understand. Thank you very much.

[ritadecassiacavaglierimede]

I loved. It was very helpful. Thank you so much.

[yahyasepahi]

Thank you so much. It was a very useful video.

[rsggho]

On 31:46 why are the colours that corresponds to the bases different ?

[pietgodaard]

Need miseq. Arrived here for lore. Satisfied

[willie]

Does anyone know how only the reverse strands are removed from flow cell?

[mikewheeler]

how is cluster density quantified? I know it is given in units of K/mm2. How are the clusters detected initially before SBS starts?

[leifang]

Back to the second question asked in the video, so if there are 5 or more Gs appeared in the first 5 images, the system won't tell if there is a missing spot for cluster or a G for the 6th read, right?

[usmanasghar]

Great video.
Can we get PPT of this lecture.

[wormball]

How do you attach different adapters to either end of the fragment?

[saabitrishrestha]

Thank you. Is it that we must always use barcoded primers (instead normal primers) in PCR for Illumina NGS?

[wormball]

Why not place index sequence after the primer and sequence it at once?

[Bob_Adkins]

This is for bacteria only, correct? Is there a similar library and detection process for viruses?

[ds]

Oh man - no dice. Anybody else here for the data analysis only? - Data Analysis is NOT discussed in this otherwise great seminar :)

[withramya]

The second question was not answered. The question was "How will the system differentiate a deletion in the sequence vs G calling (since both will look blank)?" and not "How to differentiate between lack of signal and G"- which is what he answered.