3) Next Generation Sequencing (NGS) - Coverage & Sample Quality Control

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1. Coverage 1:01
2. Sample Quality Control 2:15
3. Quantitative Validation 3:19
4. Qualitative Validation 4:48
1) Introduce gel into the chip and pressurize it
2) Load markers, ladders, and samples onto the chip
3) Vortex the chip
4) Load the chip onto the Bioanalyzer

eunseo
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Thanks for yet another interesting video!

dufo
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Very informative video.
It would be great if you could please explain which factors affect the coverage during sequencing and also how?

khushalipatel
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How can we relate the protein fragments in a cell with the DNA sequencing? If i have protein expressed under some stress in plant..how can i know the RNA sequence or DNA sequence related to the protein..

chakri
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I have an assignment(experiment to perform next generation sequencing on 10 patients with a healthy liver and 10 patients with a liver that has a genetic disorder and write the difference between their gene expression.and what will be the outcome of next generation sequencingDon't know where to start

tsikosophia
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Can you please update me what is coverage and reads?

santosh