But what is CRISPR-Cas9? An animated introduction to Gene Editing. #some2

This CRISPR animation visualizes how the CRISPR/Cas immune system was identified in bacteria and how the CRISPR/Cas9 system was developed for genome editing. This CRISPR animation also covers the history of gene editing, including zinc finger nucleases (ZFN), transcription activator-like effector nuclease (TALE nucleases, TALEN). A history of Jennifer Doudna, Emmanuelle Charpentier, Francisco Mojica, David Liu, and their contributions are described, such as the development of base editing and prime editing. We also go beyond just cutting DNA and discuss how CRISPR turns gene expression on (CRISPR activation, CRISPRa) and off (CRISPR inhibition, CRISPRi) to fluorescently tagging particular sequences (e.g. GFP). This animation explores some of the exciting possibilities of CRISPR-Cas9 biotechnology, including designer babies, genetically modified humans, the end of diseases, and genetic engineering.
But what is CRISPR-Cas9? An animated introduction to Gene Editing.

This video was made by Harrison Ngue, student at Harvard University, as the final project for the course Chemistry 177: The Chemistry, Biology, and Societal Implications of Genome Editing, taught by Professor David Liu and Aditya Raguram. Special thanks to both for providing feedback on this video!

Contents:
0:00 What is Gene Editing?
2:35 Discovery of CRISPR
5:31 CRISPR-Cas9 Technology
7:01 PAM Sequence
8:03 Modern Gene Editing

These biology animations and chemistry animations are largely made using Adobe Premiere Pro and Adobe After Effects, along with the 3blue1brown custom Python library, Manim. Icons, drawing, and chemical structures were designed using BioRender and ChemDraw.

Music:
Pure Water by Meydän
Forever Sunrise - by Jonny Easton
Check out his channel

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Powerhouse of the Cell (formerly Primo Science), founded by Harrison Ngue, aims to teach science using animation and a visuals-first approach. If you want to stay posted on new videos, subscribe.

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[ObamaBinLaden]

That _"maybe that scientist who will accomplish these feats in the future is watching this video right now."_ was super motivating to me. Thanks!

[tzftpow]

I'm a Korean high school student who was searching for terms used in gene editing and I was getting tired. BUT I was so lucky to watch this video and learn much about the overall knowledge of this field. You were just integrating all seperate information I was studying myself. This video is literally "Perfect". Thank you for making and sharing this high quality video. It was so helpful to me

[podunkest]

While this has definitely gotten a lot of attention and recognition, including a Nobel prize, I still feel like this is such a monumental discovery their names should be household names up there with Darwin, Einstein and co. It's one of those things that you'd never figure out yourself in a million years but is so elegant and conceptually simple to grasp once it's shown to you.

[kedarnavsariwala]

Dammn that last sentence "maybe that scientist who will accomplish these feats in the future is watching this video right now." felt really emotional for some reason. Thanks

[mrighakalra]

This has been so far the most creative science video I've come across. You're literally genius for putting the whole concept in such a perfect sequence and narrating it so gracefully. You literally broke those stereotypes about how science ain't easy to understand. I mean this really is Exceptional !!! Thank you soo much for such amazing efforts 💖

[jarniwoop]

I've been reading about CRISPR's accomplishments for years, and it is nice to read a good description of the process. I did have to watch it twice.

[roopesh]

This is the great explanation of crisper gene editing I've ever seen 👏

[lukassaezalmendras]

I've never seen anything related to CRISPR-Cas9 and I have to do a presentation about this method applied to crop improvement, based off of a paper. I wasn't understanding anything until I found this video. It really made the method so clear to me. Thanks a lot!

[zqxzqxzqx]

I'm just a housewife and grandma who's been fascinated by CRISPR Cas9 since the beginning. I even read Code Breaker. I'm so excited to now hear that this is being used to cure sickle cell, blindness, and deafness. Amazing.

[Ryan-nhmt]

I know that I'm late to the party, but WHY IS THIS NOT MORE POPULAR. These videos are ELITE. #1 🎉

[JohnSmithXL]

It's always nice to find a science channel that doesn't put me to sleep, keep up the great work!

[biopharmmarkets]

I love how visual these videos are. This really helps make the abstract relatable

[hanknew]

Utterly fascinating and presented with outstanding clarity and thought. Left me wanting more & more! This channel has some of the most well produced content right now, thank you for the effort and care you put into its creation

[carlosheaven]

M a fourth year biomedical student. The Clustered regularly interspace short palindromic repeats Cas9 system is my research topic and having watched this video m totally 💯 of an A+. Huge fan of your work Sir. The molecular biology content in this video is incredible .

[nicoleminotti]

Most understandable and clear explanation of CRISPR. This makes me want to take Chem 177! And I had no idea David Liu's lab is doing this cutting edge research...amazing. CCB is so cut off from what the Medical School and Broad labs are doing. :(

[huanzhang]

This is the great explanation of CRISPER I've ever seen. And the last words are perfect answer to the query of gene editing. Science doesn't stop, and technology continues to improve. Thanks to the all creative thinks and collaboration of hundreds of scientists arcross the world."

[davidrosen]

Best explanation and animation of CRISPR I've seen. Thank you for putting this together. It's been extremely helpful.

[pikulicluka]

Awesome stuff, I just discovered your channel and I'm stoked to watch all your other videos too. That was a great explanation of how CRISPR-Cas9 works!

[akshayaasatheesh]

This video is absolutely phenomenal and inspiring too. Thanks for explaining the crispr tech crystal clear and with the end note was so motivating. Great job!

[dlaminisiyabonga]

Its your closing statement for me❤ thanks for making this “complex technology” much easier to understand.