CRISPR Cas9 - A Brief Introduction

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*Spanish subtitles also available

CRISPR Cas9 system is a versatile and robust genome editing system with showing success in various model organisms. In this video, you will be introduced to this technology and its various applications and its impact on genome engineering.

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[melatgebru]

This is the best video on CRISPR system, thanks a lot!

[vasiliyabaev]

An excellent video! Saved me at least one hour of reading in 6 mins.

[iraqi]

Thank you very much Doctor but there is a bit of a problem with the sound

[palfers]

I'm having some issues with the basic CRISPR mechanism in bacteria:
1. "The invaded cell recognises the foreign DNA and stashes away a snippet of it in the CRISPR structure".
So if the cell can do that, why can't it simply chop it up and be done with it?
2. How does the cell survive the initial attack, whereby gazillions of virus copies are created and then expelled from the cell, usually accompanied by cell death due to membrane rupture?
3. How does the RNA template inside Cas9 mate up with the DNA? RNA has uracil instead of thymine!

And for genome editing, Cas9 requires a PAM match before it attempts an RNA match. This would appear to be a very strong restriction on where edits can be made. So
4. Can the Cas9 PAM be made programmable, thus allowing edits to be made anywhere?

[pohsstudio]

Thank you. I'm doing a project on CRISPR-Cas 9 in the role of drug discovery. Can you explain further how personalized medicine can be made from this system?

[drrakeebahmad]

Thanks for very nice and well demonstrated video lecture on CRSIPR Cas-9 mechanism and its novel applications.
Once a Bacterium is infected by a Bacteriophage, what type of enzymes cleave the viral genome within bacterium, to be remembered by Bacterial genome for recognition in future by CRISPR-Cas9 complex. I mean a detailed mechanism of Adaptive immune system adapted by Bacteria.

[kaleesiking]

Wondering if it can change the DNA? And if the DNA is replaced by the synthetic RNA?

[larryprice]

How long will it be before it's available for the public so I can cure diseases and help people walk again I would appreciate an answer thank you

[yashir]

Can I know if, this video is still accurate in terms of CRISPR development untill today. I mean is this all, or it has grow to have more systems than the 3 shown in the video.

[weeandyforlife]

hey, just wanting to know if there are any environmental risks to this procedure? great informational video though

[ronaldbarrett]

This is excellent genetic research. Thanks.

Can you please explain, how this system is helpful in bacterial immune system.

[mountain]

Very well explained, Can you please give your source of information so I can use some of this material and have the chance to reference it. Thanks.

[brunnosantos]

Excuse me... what do you mean with "Since mismatches at the 5' end of the gRNA are tolerated"?

[tushar]

Hi, This is a very promising concept.
Is it a theoretical/Laboratory work? OR is there a medical procedure available for patients? I will be very thankful. I have ankylosing spondilitis and HLAB27+ Thanks

[kushagrjolly]

just started working on CRISPR CAS for generating low phytate soybean

[riusengupta]

Would a CRISPR/Cas9 mechanism work efficiently in quadruplex DNA or any DNA sequence having a non-canonical or secondary structure?

[mustafamoslim]

Hi
What’s the best references for crisper cas 9 at the moment

[zahrashiri]

Thank you for the great video and informative Q&A. I was wondering if this technology can be used to introduce an opsin to mammalian cells?

[racheljesudasan]

Will a single gRNA work for all the genes, as in the all in one vector? What are your thoughts on the use of two targets for the same gene to delete the intervening sequence?