4) CRISPR Cas9 - Gene Regulation with dCas9

The CRISPR Cas9 system has revolutionized the field of genetic engineering with its simplicity and versatility. Now, scientists have cleverly created a catalytically dead Cas9 (dCas9) that is able to bind its target with high specificity without cutting any DNA. This new CRISPR Cas9 system expands the field a step further as a novel new tool for reversible gene regulation. As you will learn in this video, dCas9 can act as a modular platform upon which activators or repressors can be easily fused and targeted to the gene of interest.

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[fortitude_ra_kolahal]

The best explanation I have found so far.

[keirajohnson]

This video has been extremelly helpful for a project I'm currently organising by myself. Thank you so much!!

[rahulbasu]

Fantastic, brief and awesome video, I was trying to understand the Crispr A system, it was so nicely explained here. Thanks a lot!

[kiedranFan]

Additionally the repression/activation protiens can be engineered to bind to an extention of the guide RNA's so that multiple reactions can take place simultainously. The dCas9 shown here would only be good for one reaction at a time, where all genes targets are activated or repressed but cant do both for different sets of gene targets. RNA binding components are able to do that though and is also an option.

[ZK]

So informative yet so simple. Many thanks to the designer and the narrator.

[-io]

You help me understood the process of some articles. Plz make more vdo especially on how to analyze the results in the aricles as well. Thank you.

[nickl.]

This is an awesome video. Thank you so much.

[rahulbasu]

PAM is located opposite strand compared to the strand where guide binds.

[francestorreysirdevan]

Superb video!! Thank you so much for such an excellent explanation!

[ShortsOfInsight]

As far as I know, spacer sequence (in sgRNA) goes and binds to the DNA strand, opposite to the DNA strand that contains PAM sequence. in the video it shows the other way around. Could you please correct the video or me (If i'm mistaken).

[alvinreyes]

For example, I discovered a bacterium producing an anti-quorum sensing compound. How to use CRISPR technology to explore the gene involved? I hope for an immediate response for this question. Thank you very much.

[namrata]

extremely helpful, thank you very much.

[dubstepXpower]

How do you get the cas9 gene, and attach it all into one plasmid? Can this only be used in vitro, not inside an organism?

[andries]

Thanks for this simple overview. In the video and other videos, people call the dCas9 a reversible system, but they never explain how one removes/prevents the crispr cas9 system from working to reverse the effects? Either a simple explanation or some terms or method names I could look into?

[larryprice]

why can't they use crisper cast 9 to cure diseases help people walk and improve life and keep people dying and they HIV and stuff like that and muscular dystrophy and forget about designer babies and all that stuff that's them it could help us out so much please hurry as fast as you can keep me updated thank you