CRISPR Cas9 vs. Cpf1: 5 ways the Cpf1 nuclease outsnips Cas9

What's the difference between CRISPR Cas9 and Cpf1?
➜ Dear fellow biotech lovers, thanks for watching our first episode of Molecular Minutes! In this episode, we introduce you to Cpf1, a new CRISPR nuclease discovered by the Zhang lab which we'll soon make available over here at abm. Cpf1 is smaller than Cas9 and targets complementary areas of the genome, making it an excellent addition to the CRISPR dream team. Watch our video to learn more about why Cpf1 deserves its own fan following!

For more info on the CRISPR Cpf1 or Cas9 system and for a list of the sources used, please visit:

Want more tips, tricks, guides, and protocols on how to knockout a gene using CRISPR? Join our free, 4-week #CRISPRCrashCourse:

*Note: For those of you who may have turned off annotations, please be aware of the error at ~1:32 mins: trans-activating CRISPR RNA is abbreviated as "tracrRNA" not "tRNA". Our apologies!

Check out our other video series:

Connect with us on our social media pages to stay up to date with the latest scientific discoveries:

[anonymoushy]

I do think that Cpf1 has a lot of potential because of its sticky end cuts and that it doesn't need cRNA.
However, because Cpf1 is pretty new, while Cas9 has already been around for years, so perhaps people don't want to use Cpf1 over Cas9 for now since that would mean a lot more research and trials. But who knows, I hope Cpf1 gets more interest in future.

[JorgeRamos-xwdy]

CRISPR Cas9 is in many late stage studiesand if and when approved their technology will get an immense boost. There will always be newcomers but the true and tried can not be easily swept aside.

[jumpingpools]

Love the dad jokes. Thanks for the info, it's been helpful!

[rakibalmahdi]

can somebody explain between T-rich PAM sequences and G-rich PAM sequences?

[jiji-fj]

good video i can understand the cpf1 more thank you

[AdolfsTimrots]

Good video with a great potential. It would be nice if you got better microphone. Keep it up!

[salarahmad]

wow brilliant thanks mam please give me any good advice about study i would be thankful to you for that.

[rakeshhrx]

Hello I'm rakesh from India, I'm a muscular dystrophy paitient, can you say me whene it's start in human body ???

[mikebenstead]

Who, if anyone, monitors and regulates CRISPR usage?
What's to stop this tech falling into the wrong hands?