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Western Blotting - Principle, Procedure and Applications
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This video explains about Western Blotting - Principle, Procedure and Applications.
Link to SDS-PAGE:
Western blotting / Immunoblotting is an analytical technique used to detect a target protein in a sample (containing a complex mixture of proteins) by using a polyclonal or monoclonal antibody specific to that protein. The mixture of protein can be separated based on their size by poly acrylamide gel electrophoresis, The protein bands are transferred to blotting membrane, the most widely used membranes for western blotting are Nitrocellulose and Polyvinylidene fluoride (PVDF) membranes.
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Link to SDS-PAGE:
Western blotting / Immunoblotting is an analytical technique used to detect a target protein in a sample (containing a complex mixture of proteins) by using a polyclonal or monoclonal antibody specific to that protein. The mixture of protein can be separated based on their size by poly acrylamide gel electrophoresis, The protein bands are transferred to blotting membrane, the most widely used membranes for western blotting are Nitrocellulose and Polyvinylidene fluoride (PVDF) membranes.
Related Videos:
---------------------------------------------------------------------------------------------------------------
Designing qPCR Primers
How to check Oligo Concentration
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