TOP PRIMER DESIGN SOFTWARE TOOLS for PCR & Sequencing | Ultimate Guide

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Discover the best software tools for designing primers in this guide! Whether you're working on PCR, qPCR, or sequencing, this list of the top primer design software tools will help you optimize your experiments. Learn about popular options like Primer3 and NCBI Primer-BLAST.

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Anytime you need to copy DNA, whether it’s for diagnostic assays, forensic analysis or gene cloning, you will need to design primers. PCR or cloning primers guide polymerases to amplify gene-specific sequences. They are designed to flank the target region (region to be copied), to allow DNA synthesis and replication to occur.

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