How to Inoculate Grain Spawn Bags

This video I show you how inoculate a grain spawn bag.

Supplies List
- Grain Spawn Bag
- Syringe with Spores
- 70% Isopropyl Alcohol with Sprayer
- Alcohol Prep Pad/Wipes
- Lighter
- Gloves

0:00 Intro
0:10 First turn off A/C and furnace to minimize airborne contagions. Also try to use a room with minimal airflow and closed doors.
0:20 Next disinfect and sterilize everything!
0:42 Then prepare your spawn bag. Look over the bag to make sure there is no damages or holes. Then carefully pull open the sides of the bag to allow the filter to breathe during colonization. Use an alcohol wipe to sterilize the black injection port.
1:29 Now prepare the syringe. Shake syringe to separate spore cluster. Remove the needle and safety cap from the syringe and attach the needle tightly without touching the needle. Then flame the needle for 15 to 20 seconds and wipe to remove soot.
4:23 Next slowly inject the syringe into the injection port and inject spores in 4 to 5 places in the bag.
5:09 Finally let the bag sit in a dark warm place for about a month to allow your mycelium to fully colonize.

Check Out My New Channel Ashley Shroomer and Please Subscribe! New content coming soon!

FREE Mushroom Growing Guide!

66Qt Monotubs & Dry Bulk Substrate Kit

Dry Bulk Substrate Ingredients Kit

66Qt Monotubs for Sale!

Follow me on instagram: boomershroomer.v2

My Camera Equipment

[toddsimmons]

It’s nice to hear someone who doesn’t sound like a burn out showing how this is done. Great video! 👍🏻😀

[MaggieF]

Been watching your videos for a few months, and just bought one of your monotubs off of Amazon. Inoculating today. Thanks for the no-nonsense, simply explained videos.

[eeelblunto]

THANK GOD I FOUND YOUR CHANNEL! I ate some shrooms recently and I loved the experience so much that I had to give growing them a try. I am an amateur and for only having taking shrooms once I am both eager and motivated to harvest some of these beautiful things! Thank you for the detailed video and clear instructions on how to get going! Much love.

[Piesoplenty]

Hands down the best, most thorough step by step instructional video I've found in the mushroom community. I have subscribed.

[jenmdawg]

Fantastic! I am so glad I found your channel. I finally feel confident enough to innoculate my first grain bag and start my first grow after watching this video.

[mattthomas]

Needles are gamma sterilized, so rubbing alcohol and flame cannot improve sterilization. Those steps can be omitted. When injecting, rather than pulling the needle out and re-inserting 3-4 times at different angles, just insert the needle one time straight in, inject a tiny bit, then manipulate the needle from side to side without pulling out of the injection port. Pulling out and re-inserting risks contamination. Good video. How was your flush?

[danielalt]

When it comes to desinfection alcohol, make sure to read the manual on how much of it must be applied. In hospital we must soak everything so wet it takes about 5 minutes for it to dry, or else there is a chance bacteria has not died.

[cg]

My 1st Video I think I’ve watched of yours. Thank you so much for the great narration.
I feel like I’m in A classroom again😅 only this time I’m awake and paying attention.

[JubeProductions]

Your videos gave me the motivation to start a bulk grow using a grain spawn bag and monotub. I just got my grain spawn bag and I will be inoculating it and I wanted to watch this one more time.

[IsedaAE]

This was exactly what I needed to see before starting my kit! Thx a million!

[MrDannyd]

I appreciate the nice video with narration. Although there are a couple of things that seem odd for someone that did sterile environment work in college.

-The flame sterilization of the needle doesn’t make sense unless you are concerned that you grain bag is already contaminated: the port, grain, and needle are clean. I think this is more important if you are cutting agar as you don’t want to transfer between plates or stratification for contamination. SIDE QUEST: the soot is sterile.

-Spraying the needle is odd, as using good sterile technique will be more effective (placement of items, moving left to right, not breathing on the specimen, etc.)

[greymort]

I just started my first bag. I bought your tub kit and am very excited to see how they turn out.

[dianebryant]

THIS is the most detailed video ever, THANK YOU! I have watched many and I am so glad I came across yours 😄

[uncoolasmr]

Great vid! 1 suggestion, after flame sterilizing the needle, there's no need to whipe with alcohol. By doing so, you are basically downgrading the sterility, since you are going from sterilized to just sanitized.

[stevelove]

Thank you! I found your tutorial really instructive! Fingers crossed for me and my first attempt!

[pyrojojo]

Thanks Boomer for all your good lessons, we appreciate it!

[nosferatuzodd]

Just inoculated inoculated my first spawn bag. Blue oysters. Can’t wait!

[andyballenger]

Very informative guide. Really curious to see how they progress. Thanks for sharing!

[GloUpCosmo]

I'm waiting on my Grain Bag to come in the mail and I'm super pleased with this video

[unclesam]

thanks for the fast and detailed instructions ❤