Hemocytometer calculation

In this tutorial, we will explain how to calculate the viability, the cell density, the total number of cells and the volume you need to add if you're going to resuspend/subculture.

Enjoy!

[hypeviews]

great video...will watch this over and over until I master this formula...thanks again

[icedsolid]

Great Video. Was the most helpful I've found for a beginner. My only question is if the volume per square (0.0001ml) is consistent across hemocytometers and if a micropipetter is require for measurement of liquid into the hemocyctometer.

[beboweke]

thank you very much, best explanation ever

[yrs]

Thank you very much can you elaborate how to consider volume of a square for cell count?

[judestarz]

Thank you so much for this Video, it was very useful.Just a quick question, how can I calculate the average number of cells if I counted the cells in only two small boxes from each of the large 16 square corner boxes? So, I counted two boxes in each four large boxes. I have 8 values in total. Do I multiply the sum of each two boxes by 8 then add the vales and divide by 4?

[marwafaheem]

It's a great job. But I would like to ask, according the final volume which is mentioned to be 28.75 ml, this the volume of fresh medium to be added to the cell suspension or this is the final volume of the cell suspension need to reached

[danaal-azzeh]

Thanks for a great video. I have one question, if we are subculturing the  volume that you calculated as total cells/target density (cells/ml)  represents the volume we take from the cells ?

[armendmurati]

Hi, I am Armend from Pristina, Rep. of Kosovo.
I have a question. When you calculated the final volume, in a first case as a target density of cells you took number 200.000 cell/ml, and during the count of eritrocites in the medium square with 5 fields counted, the value of target density of cell was 2.000.000 cell/ml. Where did these two above mentioned values come from during finally volume calculation?

I have a question. If I want to subculture 0.5x10^6 cells/mL, what is the final volume that I must take from original cell suspension (original volume=15 mL) with cell density 0.7 x10^6 cells/mL to make a new suspension with total volume of 15 mL?

[shajidislam]

Thanks..but i have a question..you tell us follow 4 square or only middle square ...what should we follow

[elenavillacorte]

I have a question, if the volume of a one corner square is 0.0001, why the value for the volume of the square is 0.0001 @01:24, should it be .0004 ml? (0.0001x4 corners)

[soadbohorquez]

great video, very helpful. Can you please explain to me what the 0.0001 mL means? in my cell bio notes I have is in scientific notation as 10^4grids/ml. Thanks

[MrIcemaann]

Good Video. But you cant have a dilution factor of 1:1! I think you meant 1:2 because that means you have 1 part of cells and 1 part of Dy Solution

[lemeza]

I am a bit confused when using dilution factors, can you please explain this? It seems so simple yet I can't understand it!

[alebraheme]

In the next case, why you divided by 4*10^6 not by 10^4

[amagolan]

talking way too fast! I had to keep pausing it to take notes. I know you wanted it under 5 minutes, but that was way too fast

[biolabprotocols]

I'm sorry this is the case for you. If you have any questions, I'll be happy to answer them here. I also do take feedback very seriously, so I would appreciate it if you tell me specifically what you feel makes it confusing so that I can make it better.

[ChrisYangDOC]

so we are not calculating the dilution factor as in chemistry rght?? its different here nah?

[raahul]

Guys just reduce the playback speed to 0.75x and it'll be perfect

[aramvartanian]

even more confused than i was before this video