Restriction Enzymes

Video used for teaching on module 500709 Cellular Regulation and Biotechnology at the University of Hull

[hellfrr]

Thank you so much for posting your videos. Very very clear explanation, great for understanding and for easy review later. You're saving me a lot of time and confusion. You're a wonderful woman

[Encrypted]

I think I am in love....

I totally enjoyed this lecture.

For the cloning strategy you explained how to put the sticky ends onto the GOI by using PCR, however, you did not explain how to include MCS onto the plasmid.

Could you kindly shed light on that please?

Thank you!!😍😍😍

[damlagumren]

Thank you for all your support! Great video !!! <3

[lionardl]

Hello, if we have one EcoRI site and one EcoRV site how do we put the insert if it have the same endings that EcoRV but not EcoRI?

[ruggerogabbrielli]

Did bacteriophages evolved not to have those palindromic sequences?

[solomonderese]

I have a problem with your use of two different restriction enzymes to cut the palsmid and gene of interest, won't this affect the joining of sticky ends through hydrogen bonding before they are ligated?

[RobertAndersch-jg]

Hi is there wobble in the recognition of the restriction site