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Restriction Enzymes
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Restriction enzymes bind to specific short sequences (usually 4- to 6- bases long) on the DNA. The nucleotide sequence on both DNA strands is identical when read in opposite directions. Most restriction enzymes cut the DNA in a staggered fashion. This generates “sticky” ends. These ends can pair with any other DNA fragment generated by the same enzyme. The pairing is aided by DNA ligase.
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