Restriction Enzyme Digests

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A brief introduction to restriction enzymes, followed by a demonstration of how these enzymes can be used to cut DNA and then analyzed on an agarose gel.
  1. Jacob Elmer
  2. restriction enzymes
  3. digests
  4. agarose gel
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Комментарии
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Excellent explanation!! I was having lots of doubts regarding restriction digestion this video cleared my doubts completely!!
You are a very good teacher! Thanks a bunch!

lona
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First time doing this experiment and I totally understand very, your explanation was fantastic

tryhard
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Can you please help with the calculations; I have 20000 unit/ml restriction enzyme, how to calculate for 20 unit out of it?

Its_InduB
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I have some questions:-
Can you tell me the exact volume of all the reagents u used in this experiment including water and buffer?
What is buffer 2.0?

Its_InduB
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what is the percentage of your agarose gel? I read that we need to have it at a higher concentration to see the separation

ForensicNA