Restriction Enzyme EcoR1 (2000) Drew Berry wehi.tv

[deirhese]

Dark Pink = Vector (Plasmid)
Bright Pink = Foreign DNA fragment (attaches to the vector which is the plasmid)
Blue = Endonuclease (cuts DNA at specific sequences)
Green = DNA ligase (binds sticky ends back together which makes DNA stable again)

[xZanthophyll]

why is it so unnerving yet informative 😅

[petergambier]

If only we'd had Drew Spielberg's medical animations when I was at school, swear to god, I'd have been more keen to learn science stuff but they never even had computers in those days.

[povilasqw]

I'd rather play slender in middle of forest in moonless night than watch this before sleep

[ZAZA]

My friend was right, this is scary...

[DanielHHowell]

This is the greatest and cutest enzyme of them all!

[khy]

I clicked on this to learn, not to shit my pants. Holy shit.

[regulate.artificer_g.mdctlsk]

I was surprised to see that there was a Vinny Vinesauce meme made out of this. To me that means that either Vinny, part of his fanbase, or both, were into WEHI molecular biology animations, and that makes me proud to be part of them.

[Kristen]

This is super scary ... and it kind of grossed me out for some reason...

[sesshysayoyasha]

Wow, that was really helpful. Thanks.

[burlappsack]

so the overhangs just fly right into eachother seconds after cleavage? i think this video fails to explain the chaotic nature of brownian motion, but is helpful none the less

[Thuynh]

A restriction endonuclease (Blue blob) cleaves the recognition site in which a palindromic sequence on the top and bottom strand exists. Ie: ATT - TTA. The extra piece of DNA (also cleaved elsewhere) is attracted to the cleaved end because it's sequence is complementary to endonuclease cleavage. Ligase (green blob) bonds the strands together and successfully completes transformation.

[panesar]

the geen one is called DNA ligase, which joins the DNA strands.

[AskScience]

I have no idea. I believe blunt ends can still be used simply because one end is a 5'po4, and the other end is a 3'oh so the ligase will bind those togather..

but I can't be certain. You should email a molecular biology prof.

[AskScience]

Here is my Commentary

it is a dna molecule.

at 13th second, the blue coloured being is known as a restriction enzyme. these are enzymes that find a specific site on teh dna molecule and cut it. It makes staggered ends.cutting process requires a Mg molecule and it produces 5' PO4 end and a 3'OH end.

this end is recognized by a Ligase. this is an enzyme that can join the two ends togather. two of them are needed for two strands and thus we have successfully placed a dna fragment into a plasmid.

[bobofunnyrabbit]

Is there an actual footage of dna, etc? Or are we limited to CG animations only?

[lrissunflower]

Why did it attach the foreign DNA to the vector? I thought restriction enzymes cleaved foreign DNA to protect the plasmid/ vector

[kaviyarasisivaraman]

OMG excellent animation.. thank you very much

[AskScience]

because when you got your little piece of dna that you are trying to add to a plasmid piece of dna, if you cut both pieces using sticky ends, then you can use just put them togahter in a mixture and they would automatically bind togather, but its much harder to join blunt ends togather because they have no need to join togather.

when you use sticky ends, the two pieces wanna get togahter becuase they are complementry with each other = hydrogen bonds = more stable.

blunt ends have no need

[wendijoyce]

the pink one is the vector? cleaved by the blue one, which is the restriction enzyme? the green one, which binds the new DNA fragment to the vector is called??

:) thanks, nice animation still.