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The invisible dance of CRISPR-Cas9
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The movie shows in real time the process of activation of the CRISPR-Cas9 genome editing tool. Molecular simulations reveal that the activation occurs through a fascinating process by which the protein and the nucleic acids are "sensing", "regulating" and "locking" the catalytic HNH domain.
Read more about this mechanism in the original paper:
G. Palermo, J. S. Chen, C. G. Ricci, I. Rivalta, M. Jinek, V. S. Batista, J. A. Doudna and J. A. McCammon. Key role of the REC lobe during CRISPR-Cas9 activation by “sensing”, “regulating” and “locking” the catalytic HNH domain. Quarterly Review of Biophysics 2018, 51, e9.
Read more about this mechanism in the original paper:
G. Palermo, J. S. Chen, C. G. Ricci, I. Rivalta, M. Jinek, V. S. Batista, J. A. Doudna and J. A. McCammon. Key role of the REC lobe during CRISPR-Cas9 activation by “sensing”, “regulating” and “locking” the catalytic HNH domain. Quarterly Review of Biophysics 2018, 51, e9.
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