DNA replication in prokaryotes 2 | Prokaryotic DNA replication elongation

DNA replication elongation in prokaryotes- This DNA replication process lecture explains the second stage of DNA replication process that is elongation and DNA synthesis in prokaryotes. The step-by-step process of DNA synthesis by DNA polymerase enzyme with the help of leading and lagging strand of DNA is explained in this video lecture.
DNA replication will be the procedure for producing 2 equivalent reproductions collected from one of first DNA molecule. This kind of natural method takes place in all residing organisms which is the basis intended for natural monetary gift. DNA comprises of 2 strands in addition to each and every follicle from the first DNA molecule acts being a web template to the manufacturing from the secondary follicle, an operation called semiconservative replication. Cell phone proofreading in addition to error-checking things ensure around great fidelity intended for DNA replication.
The moment priming will be comprehensive, DNA polymerase III holoenzyme will be packed into your DNA along with replication commences. This catalytic process involving DNA polymerase III entails using a pair of metal ions inside the lively web page, plus a region inside the lively web page which could discriminate concerning deoxyribonucleotides along with ribonucleotides. This metal ions usually are basic divalent cations that will guide the 3' WOW begin a new nucleophilic attack onto the alpha dog phosphate on the deoxyribonucleotide along with orient along with support the badly priced triphosphate about the deoxyribonucleotide. Nucleophilic attack by the 3' WOW about the alpha dog phosphate produces pyrophosphate, that's next subsequently hydrolyzed (by inorganic phosphatase) in a pair of phosphates. This specific hydrolysis pushes DNA synthesis for you to achievement.

On top of that, DNA polymerase III ought to have the capacity to distinguish concerning the right way used basics along with improperly used basics. This is attained simply by distinguishing Watson-Crick bottom twos using a dynamic web page wallet that may be contrasting in form towards the composition involving the right way used nucleotides. This specific wallet incorporates a tyrosine remains that will has the capacity to kind vehicle der Waals relationships using the the right way used nucleotide. Also, dsDNA (double stranded DNA) inside the lively web page incorporates a larger important groove along with shallower minor groove that enables the enhancement involving hydrogen bonds using the 3rd nitrogen involving purine basics as well as the next fresh air involving pyrimidine basics. Ultimately, the lively web page makes comprehensive hydrogen bonds using the DNA central source. These types of relationships lead to the DNA polymerase III closing all around a new the right way used bottom. If a bottom will be placed along with improperly used, these kind of relationships couldn't arise on account of disruptions within hydrogen developing along with vehicle der Waals relationships.

DNA will be understand inside the 3' → 5' route, consequently, nucleotides usually are synthesized (or attached to the theme strand) inside the 5' → 3' route. However, one of several parent strands involving DNA will be 3' → 5' even though the other will be 5' → 3'. To unravel this specific, replication occurs within reverse directions. Going towards replication branch, the cutting edge strand will be synthesized in a ongoing fashion, merely requesting a single primer. On the other hand, the lagging strand, proceeding away from the replication branch, will be synthesized within some small fragments known as Okazaki fragments, for that reason requesting quite a few primers. This RNA primers involving Okazaki fragments usually are subsequently degraded simply by RNase H along with DNA Polymerase I (exonuclease), as well as the holes (or nicks) usually are stuffed with deoxyribonucleotides along with closed by the enzyme ligase.
DNA replication by Suman Bhattacharya
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[Manisha-mfqe]

Thanku Bhagwan ji💓🌼😇🌈🤗🙏Thanku sir🙏😇🌈🤗💓🌼

[sofiaelectra]

Instead of only pointing out the minor mistakes in this video, you could also praise this man for explaining difficult subjects so easy for us to understand. Keep up the good work and we will keep the A grade at the biochemistry!!

[rachanadaschaudhury]

Lucid explanation. But there is a small correction I would like to add. The direction of the last primer of lagging strand will be reversed(i.e., 5'--> 3'). :)

[pratikonkar]

Your video's are very knowledgeable as you go deep into the concepts and mechanisms. Love to watch and gain knowledge from your videos.

[shido]

with your explanation, this mind blowing topic become so easy to understand...thank u for such great content to reply on.!!!

[komal]

Can't believe it is 7 years old video, 😮so much detailed explanation ❤❤ thanks sir

[kaoutharnajim]

Perfect Sir!! You resolve a huge problem I had nerver understanding how the process goes, and now I understand it! Thank you, Thank you, Thank you!
May Allah bless you, secure your knowledge and your simple yet complete teaching methode!

[nilufarafreen]

ur videos r too helpful for me...when i hv confusion about any topic..i alwys search ur videos....ur speaking is also very fine...u alwys use easy words in ur sentence

[LS-ombb]

This has helped me a lot.Such a great initiative to clear out the doubts of many who are struggling like me.

[joshcaldwell]

Excellent video! You posted it in just the perfect time too. I'm studying for my Biochem final exam right now, and this has been super helpful to visualize everything!

[ОбычныйПерсонаж]

I found one mistake, but in common your lessons are helping me a lot (right now) to pass the exams for the magister program. Thank you so much for your hard work!! I found all I needed and even more. Priceless material if you're tired of reading books (temporarily), and there is a need of human touch :)

[shubhamaggarwal]

Thank you so much sir for such a informative video ...i was always afraid of this topic, but you made it like a childhood story

[nandhiniperumalsamy]

Thanku so much brother...u r a best soul...I don't have a best teacher like you....

[snehalvaidya]

Your explanation is far more better then my professors....good job keep it up

[srish]

Aatma tript ho jati h aapse pdne ke baad🙏You are a gem❤️

[bhargabjyotibaruah]

Thank you sire. You are the real gem for all life science students. Lots of love and respect from assam.

[barborak]

I don't think that is correct... in the second part of the video the primer in the loop…when you imagine you straighten the loop out... than the primers in both chains will be in the same direction….or…leading strand is 5‘ (on the left) – 3´(on the right) so lagging strand is 3‘ (on the left) than on the end of the loop it should be 5‘ ....but primer on it starts also with 5‘…Still thanks for your videos, your teaching helps me a lot!

[malihauddin]

it is soo well explained!!
Great explanation as always from Shomu Sir!
I hope I master this concept and answer all the questiona from this topic

[dolanbiswas]

Thank you so much sir
This is so helpful..
Lots of love and respect from Kolkata
Sir there is just no doubt after seeing your lectures..

[dfgange]

Thank u very much sir ...
Ur teaching method just amazing....👍👌