Polymerase chain reaction (PCR) Amplification #genomics

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The polymerase chain reaction, or PCR, is one of the most well-known techniques in molecular biology. Replication of single-stranded DNA from a template using synthetic primers and a DNA polymerase was first reported as early as the 1970s. Nevertheless, the PCR method as we know it today to amplify target DNA was not developed as a research tool until 1983, by Kary Mullis. Since then, PCR has become an integral part of molecular biology, with applications ranging from basic research to disease diagnostics, agricultural testing, and forensic investigation. For his invention, Kary Mullis was awarded the Nobel Prize in Chemistry in 1993.

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