Restriction Enzymes | Restriction Endonucleases |

Suppose We have stertch of DNA containing the traget gene to be clonned.

The central feature of restriction enzyme is that it has a particular recognition sequence at which it cuts a DNA moleule.

Here we are taking example of EcoR1 ...

So what this enzyme will do is that basically it would scan all over the dna until it finds this specific sequence.
And wherever this particular sequence is found in the dna it would cleave the dna at that point.