Restriction Digestion of DNA

This video demonstrates how to set up a restriction digest using the Bio-Rad Restriction Digestion and Analysis of Lambda DNA kit. Techniques for setting up a restriction digest using a micropipet are shown.

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[arseniipavlenko]

So no one is gonna mention that the tubes spell out HELP at the end?🤔

[beanheadr]

Interesting that this process has not changed much over the years. I did a high school internship at the Kennedy Krieger Institute back in the late 90's working with X-ALD with mice as our catalyst. I remember being nervous using ethidium bromide after my lab director explained that exposure to it could cause my DNA to mutate!

[arresteddevelopment]

Will I ever get to do this again? It was fun. Sigh

[geneie]

4ul lambda DNA
5 ul buffer (EPH)
1ul restriction enzyme
Centrifuge, incubate overnight, run gel

[Han-cpds]

what do u mean by corresponding tubes for the different restriction enzymes? this video was informational!

[rajubharti]

Is restriction enzyme added in L tube or only in E, P, and H tube ? make it clear .

[siddharthsrivastava]

Video on calculations that go into setting up such restriction digest