Restriction endonuclease vid 1

Brief overview of what nucleases are and specifically endonucleases.

[somebuddy]

Thank you so much! You've explained more in 9 minutes than my lecturer did in 2 hours!

[alextheromanian]

wish i had you for a teacher at my university.

ours just blew through this stuff under the assumption that we can just figure it out from his abbreviations and wasted the time he faught to gain by talking about how back in his day things were simpler.

it helped alot thank you!

[nadiasomething]

Wow, great stuff! Currently learning about genetic engineeering for my last year of A Level, and this has been so helpful! Will definitley look to watching your videos during uni for my biology degree this September!

[mehrshadpiano]

wow . it was so useful for me . thanks for uploading . you're a very nice teacher . awesome .

[NexusEden]

That was excellent - thank you so much. 

[rockthegrey]

That helped me alot!! Thank you! You obviously know better how to explain that stuff than dozens of book authors...
One thing left: you said, in the lab you guys really want to use the exonucleases... (at about 3:10 min)... but you meant endo, right?

Anyway, great stuff! :-)

[hamasaful]

@1992account i think he means that those 2 enzyme recognition sequences must be found also in the multiple cloning sequence of plasmid, since we should cut using the same enzyme in the gene of interest ( or on its sides ), and in the plasmid, so they match together

[gkpeter]

Your welcome. Anytime you can think of a concept you need explained, feel free to give me a request.

[suzandyve]

Can you break it down for me why you always add salmon sperm DNA or calf thymus DNA, to for instance s1 nuclease procedures???

[gkpeter]

@rockthegrey Thanks for the comment, and yes, I meant endo. Good catch, I was hoping no one would catch it. :)

[account]

Nice vid, I just wanted to ask - at about 5:00 you say something along the lines of "If i can find restriction enzymes that can flank my gene and that match up with my MCS".
What do you mean by 'match up' in this context?

Thanks.

[gkpeter]

@rockthegrey I know it wasn't meant to be picky. I am glad you caught it.

[rockthegrey]

@gkpeter It wasn't meant to be picky ;-) Just wanted to make clear. Thanks :-)

[hamasaful]

@nadiasomething what's your faculty cuz i'm studying biotechnology in egypt and i think that you seem to be egyptian or an arab from your name :D

[DrPsymotion]

@gkpeter i caught it too am also a good student right?XD

[hamasaful]

@alextheromanian lo0o0o0o0o0o0ol, exactly what's happening to me :D :D besides he's made about 50 or something papers and phds and masters , so he won't waste his time trying to explain to us even what's a rest. enz. :D

[suzandyve]

Can you break it down for me why you always add salmon sperm DNA or calf thymus DNA, to for instance s1 nuclease procedures???