siRNA vs shRNA vs miRNA | Differences | Gene silencing techniques

preview_player
Показать описание
This lecture explains about the differences between siRNA, shRNA and miRNA. These all are important gene silencing tools.
This lecture will be helpful for you to crack your PhD entrance exams. If you are looking for a school teacher job, lecturer job or want to be project assistant or research assistant, or willing to get a job in your dream Biotech company you need to have your basics very clear. This video will help you to crack your next interview. Your time is precious. Let’s grab the concept like a pro in very short time. Do not forget to subscribe my channel if you don’t want to miss a single video in this series. Your subscription will mean a lot to me.
Did you already check the following lectures?
CRISPR (Part 1) | Bacterial defence | Quick concept | Study smart in minutes
How CRISPR Cas system works? (Part 2) | Mechanism | Quick concept | Study smart in minutes
CRISPR Cas (Part 3)| Applications | Quick concept | Simplest concept
CRISPR Cas (Part 4) | How to perform in lab? | Quick concept | Simplest concept
Surveyor nuclease assay | T7E1 assay | CRISPR Cas9 screening technique | Quick and simplest concept
TALEN | Transcription activator like effector nuclease | Gene editing tool | Target specific
Zinc Finger Nuclease | ZFN | Gene editing tool | Target specific | Simplest concept
Gene silencing techniques | CRISPR vs TALEN vs ZFN | Genome editing methods | Differences
RNA Interference | RNAi technology (Part 1) | RNA mediated gene silencing tool | RNA silencing
siRNA mechanism | Gene silencing | RNA interference by siRNA
micro RNA | miRNA mechanism | Gene silencing | RNA interference by miRNA
siRNA vs miRNA | The differences | Gene silencing techniques
shRNA mechanism | short hairpin RNA | small hairpin RNA | Gene silencing technique | Gene knockdown
Learn cloning basics (INR 999/-)
Day 1: What is cloning? What is a vector? Difference between cloning vector and expression vector
Structure of a vector. How to read vector map?
What is Multiple Cloning Site (MCS)?
Steps of cloning
Day 2: How to set up a PCR reaction?
Agarose Gel Electrophoresis
PCR product extraction and purification from gel
Restriction digestion
Ligation
Transformation
Learn primer designing (INR 499/-)
Customize your primers for super successful cloning
What are we offering?
Extract DNA sequence from NCBI database
Identify CDS (ORF) from whole gene sequence
Make best customized primers for PCR, q-PCR, mutant PCR, deletion mutation, point mutation
Special offer: Get package 1 and 2 @ INR 1299/-.
Bonus: Discussion of your unsuccessful PCR in lab for free
All packages consist of full customized protocols provided in PDF
Рекомендации по теме