4.4. Next Generation Sequencing - Practice Session : Variant Calling

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Комментарии
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Hello, how do I determine the correct filtering threshold in VCFfilter? There is a minimum threshold value? Should I use 200 as a correct filtering threshold or can I use a 220 based on my first result?

camilocarias
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From a multisample vcf file
how can get only a subset heterozygous (1/2) using VCFfilter?

dylandog
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Very helpful video, thank you!! I am not really familiar with bioinformatics and in this part of my project, I am trying two compare two VCF files corresponding to the results of healthy tissue and tumor tissue. I want to compare these VCF files and remove their similarities. More specific I want to remove the information of the healthy tissue from the tumor one. Have you any suggestions on which tool I should use or any way that I can do my analysis? thank you in advance!

elenips
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Very clear explanation. Thank you very much

JenniFadoni
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Anyone else here hopeless after the coursera project? Lol

niusha
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bioinfo is overrated, lots of tools become obsolete, .

AyrodsGamgam