Gene Cloning | Recombinant DNA Technology | Video 1

Gene Cloning
You probably have heard of cloning. A clone is a genetically exact copy. It can be a clone of a gene, a cell or an organism.
Gene cloning, also known as molecular cloning, refers to the process of isolating a DNA sequence of interest for the purpose of making multiple copies of it. The identical copies are clones.
Recombinant DNA is engineered through the combination of two or more DNA strands, combining DNA sequences which would not normally occur together. In other words, selected DNA (or the DNA of "interest") is inserted into an existing organismal genome, such as a bacterial plasmid DNA, or some other sort of vector.
The recombinant DNA can then be inserted into another cell, such as a bacterial cell, for amplification and possibly production of the resulting protein. This process is called transformation, the genetic alteration of a cell resulting from the uptake, incorporation, and expression of foreign genetic material.
Recombinant DNA technology was made possible by the discovery of restriction endonucleases. loning of a segment of DNA of interest can easily be carried out with restriction enzyme digestion, followed by ligation and transformation or transfection. In the classical restriction enzyme digestion and ligation cloning protocols, cloning of any DNA fragment essentially involves four steps: isolation of the DNA of interest (or target DNA), ligation, transfection (or transformation), and a screening/selection procedure.

[siddhantaggregate]

I am in class 9 and I have watched your all the videos. Most shockingly I could understand all of them. Best teacher in simple words

[itx_khpal]

Apka study ka tariqa acha ha sir or best lecture ha inshallah sir 2 may ko biology ka paper ha iOS ma achy marks aye gy so I cleared my all dougts with the help of this lecture

[hollywoodworldandbollywood]

Aapke study ka tarika kafi Acha and simple hai kbhi kbhi unacdmy ke jesa lgta hai 😊

[bismakhan]

Your method of teaching is very simple and easy to understand 👍

[Salar-qcvh]

Who give a like to him just for the way he stay for azan ❤🎉

[Uzma_]

Thanku so much sir for uploading lectures on RDT 🙏

[hirafatima]

Thank you so much sir today was my exam I studied your molecular biology lecture 😊my exam gone fantastic 😊 🇮🇳

[rizwanamalik]

Very well explained & very impressive way of explaining the process.

[sabarkhanlectures]

Mashallah very nice, simple, and informative lecture

[mujeebkhanmujeebkhan]

Your ways of teaching is very magnificent and Adorable keep up your work further more...

[sumiyaqureshi]

Allah ap ko es ki bahtareen jaza dy❤😊

[trex-pubgmobile]

Nice explanation love from India well explained sir!!🥰

[JavedAhmedMengal]

Wow sir amazing i am big fan of your always but today you are amazing you stopped the lecture during azan god bless you sir 💓🌹

[pallavigoyal]

Best teacher thanks for clearing all the concepts

[Lilly]

All the explanations are easily, simply described .. thank you sir❤

[babydoll-ugqk]

Thannks Sr you are clear My every confusion

[MCHRiyaSharma-kwve]

Your wqy of teaching is best after watching your videos i can't forget the topic

[rahilafatima]

Notes are not clearly visible plz use bright markers

[MANGALSINGSOY-bxqh]

Sir,
Apko mere taraf se bohot bohot shukriya
Mene bohot sare lecture dekha barna samaj neme dikat huatha abhi apki lecture dekh kar concept clear 🎉🎉🎉🎉🎉

[spicysugar]

Thank you so much for this awesome lecture sir ☺️