CRISPR Genome Editing of Fungal Pathogen |Protocol Preview

CRISPR-mediated Genome Editing of the Human Fungal Pathogen Candida albicans - a 2 minute Preview of the Experimental Protocol

Ben A. Evans, Ethan S. Pickerill, Valmik K. Vyas, Douglas A. Bernstein
Ball State University, Department of Biology; Whitehead Institute for Biomedical Research,;

Efficient genome engineering of Candida albicans is critical to understanding the pathogenesis and development of therapeutics. Here, we described a protocol to quickly and accurately edit the C. albicans genome using CRISPR. The protocol allows investigators to introduce a wide variety of genetic modifications including point mutations, insertions, and deletions.

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