Introduction to Restriction Endonucleases || Restriction Enzymes

Introduction to Restriction Endonucleases - This is the video on Restriction Endonucleases which are also called as Restriction Enzymes with NEET PG Biochemistry MCQs.
It contains
Definition of Restriction Endonucleases - 00:46 minutes
Restriction Site and Restriction Fragments - 01:01 minutes
Types of Restriction Endonucleases - 01:42 minutes
Nomenclature of Restriction Endonucleases - 02:38 minutes
Restriction Endonuclease As A Molecular Biology Tools - 03:39 minutes
Sticky ends and blunt ends - 04:09 minutes
Formation of recombinant DNA from restriction fragments with
sticky ends - 04:41 minutes
Restriction Map - 05:29 minutes
Specificity of Restriction Endonuclease Enzyme - 05:47 minutes
Homopolymer Tailing - 06:31 minutes
NEET PG MCQs - 08:36 minutes

One of the major obstacles to molecular analysis of genomic DNA is the immense size of the molecules involved.

The discovery of a special group of bacterial enzymes, called restriction endonucleases (restriction enzymes), which cleave double-stranded DNA (dsDNA) into smaller, more manageable fragments, opened the way for DNA analysis.

Restriction endonuclease hydrolytically cleave polynucleotides internally at specific palindromic sites
They belong to class III hydrolases

Type of restriction endonucleases
Type 1 - Cleave at random sites
Type 2 – cleave at palindromic sites

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