DNA sequencing | Biomolecules | MCAT | Khan Academy

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[cocokat]

Can you make a video on Next-Generation Sequencing please?? It would be so appreciated!!

[MisterRazz]

@1:11 you're missing your triphosphate groups to make these deoxynucleotides (dNTPs) and dideoxynucleotides (ddNTPs).

[usmanshami]

I LOVE YOU Khan Academy you're the BEST.
Have multiple tabs opened right now doing genetics. You make the most screwed up pain in the ass topics easy as nothing ! WOW. just WOW

[phoebedu]

Thank you! I'm currently learning about this in pre-med and it left me pretty confused. This helped greatly!

[lilycarrington]

I've been struggling to grasp how DNA sequencing works, but when I watched your video it finally just clicked. thank you!

[Soulblade]

Not to nitpick or anything, what you drew up were nucleosides. Good video though...

[preciousmaelane]

Where do we get the ddNTPs and the dNTPs that we are adding which cause termination of transcription? Are they made in the lab or?

[ithirstyforknowledge]

excellent video to give a picture of what sequencing is to a newbie like me

[lucianocilio]

What if by a random chance, in different readings, the ddNTP attaches & terminates the polymerase at the same nucleotide, with consequence of generating several copies of same sequence? Would this cause confusion at electrophoresis reading? Or not because it's still the same length?

For example, if we had: AAGACATT... and at two or more times the ddGTP attach on the first G (since attachment is random and repeated a lot of times), it would end up giving two strands of TTC. Then these two be run through electrophoresis.

[parisarp]

You are really good teacher i dident understand this aces before i saw you video

[mangeshkritya]

Please can you elaborate on the last segment.
How does the computer 'by reading out the last DDNTP can sequence' can come up with the actual DNA sequence?

[languageandmana]

I wish it would be more detailed. But thank you so much

[emilopez]

my speakers aren't working well, and I wanted to know if anyone could help me out by answering my question. Where is DNA send off to get sequenced?

[alikashour]

I barely could hear you could you raise ur voice in other chapters... but anyway ur method of elination is pretty good

[damienfromAKA]

dNTP is deoxynucleotide TRI PHOSPHATE - why do you not show the phosphates?

[fadeskywards]

Excellently explained. Thanks so much!!

[fareshejazi]

i understand the method but what is it used for

[kiruthigaelango]

Where I can download cancer dna sequence dataset can anyone share link plz

[bobl]

5 minutes of awesome explanation. Thanks!

[mrLogan-sttb]

thanks, but if you type by keyboard will be good