17. Genomes and DNA Sequencing

MIT 7.016 Introductory Biology, Fall 2018
Instructor: Adam Martin

Professor Martin talks about DNA sequencing and why it is helpful to know the DNA sequence, followed by linkage mapping and then the different methods of sequencing DNA.

License: Creative Commons BY-NC-SA

[krightonzilon]

Reading textbook once isn't enough. Real sense of understanding comes after watching this video. Ossum video. Thanks MIT.

[safarscience]

Wow such a beautiful lecture and great explanation ❤

[woloabel]

(On Friday of February 3, 2023). Introduction to Biology in the Matter of Genomes and DNA Sequencing Therein: 1) Polymerase Chain Reaction (PCR); 2) Engineering A New Gene (Synthetic Biology); 3) Fusion Protein; 4) Molecular Markers; 5) Genetic Variation (Variant Inheritance Pathology Example); 6) Microsatellite; 7) Recognizing a A Unique Sequence; 8) Gel Electrophoresis (Agarose GE for DNA Sequences); 9) Other Molecular Markers; 10 Single Nucleotide Polymorphism (SNPs); 11) Restriction Fragment Length; 12) Digest Length Polymorphism; 13) DNA Sequencing; 14) Sanger Sequencing; 15) Di-Deoxyribonucleotide (Green Florescence Protein); 16) Chain Termination Method; 17) Chain Termination; 18) DNA Polymerase (Molecule of Replication of DNA); 1) Next Generation Sequencing (Curren Most Updated Methodology of Assessing DNA Sequences); PhD Adam Martin, Man muss wiederbauen und stellen Mensch aber auch man soll immer frei sein oder Sterben werden. Heil!

[lifefan]

If you don't have any question, then there are two possiblities: 1.You understood everything. 2. You understood nothing.

[voiceofreason]

In English. If you know what you're looking for, download it from a library, slit in to two strands; chuck the middle bit, find an entry cut, add your start sequence, add your rNA cut, insert your stop code. You're done.

[magnfiyerlmoro]

What is the R stuff ? in the line genomic dna

[benbirtapirim]

26:00 by just looking at the simple pedegre it can be both recessive and dominant. But when we look to the gel electrophoresis result, isn't dominant the only choice? Because it is obvious that the M'' gene is responsible for the disease, all the sick offsprings have that gene and they got that from their mother. If the disease was recessive the father should have been a carrier, which means he shouls inherit the recessive gene to the offsprings for them to have the disease. This is the only choice, but the sick offsprings was able to get different alleles from their father. A carrier inherit only one type of recessive allele. I am confused about that part🥲