How to Mutate a Sequence Using BioPython

In this video, I will explain how you can mutate and manipulate a sequence using BioPython.

In Python, we cannot mutate or manipulate a sequence because Python does not allow manipulation of a particular position of string on runtime. Therefore, we cannot change the character of a sequence.

The Bio.Seq module in BioPython provides us with a class MutableSeq(). Using this MutableSeq() class we can take the input from the user and mutate the sequence on run time.

In this code, we are first defining our sequence as a string “ATA”. Then we are using the MutableSeq object to change the variable myDNA and then we are just printing the type of mDNA variable. On execution, you can see the type of myDNA variable.

After this, we are just printing the myDNA string and in the next command, we are changing a position in the string myDNA by assigning the element “O” to the second position of the myDNA sequence. This will replace the “T” in myDNA sequence with “O”.

Then we are just printing the myDNA sequence again. On execution, you will see that the myDNA sequence stored in the string as “ATA” will be changed to “AOA”.

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-BLAST Database Searching, Parsing, and Extraction
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