Retrieving Gene & Promoter Sequences

How to look up the mRNA transcript (no introns) and putative promoter sequence for a target human gene.

[ahmadniazrahman]

This video has been a life-changer for me. I think every view of this video resulted in a scientific paper. You deserve credit for each one of them. This is a prime example of how science grow. Thankyou.

[juliablaszczyk]

You do not even know how helpful is that for me right now!!!! Very grateful for this tutorial! :) Create such videos more often

[mominamalik]

For the very first time someone made these complicated things easy for me.. thank you sooo much for such a detailed video. I really really appreciate your effort n skills. Please also make a video on primer designing for different PCR like Qpcr simple pcr thank you

[yeny]

I feel like crying from how happy I am for coming across this amazing video. I wish I could subscribe to your channel a million times.

Thank you so very much.

[jonathanhaber]

I have never commented on a youtube video in my life, but I have to make an exception. This is an amazing explanation and tutorial on finding an unknown gene sequence of a known protein. This helped me so much. Thank you!!

[Fitriananr]

Your videos are life saving! Thank you

[mahshidpooladvand]

The best tutorial in the history of tutorials!

[Viraj_]

I cantvthank you enough for teaching it in wonderful way...love from India❤

[wamiqfarooq]

I want to give you a hug, thank you so much. You are helping people.

[Kalyanraj]

These videos are extremely useful for me as a Biology student. I have recently binge watched all of your educational videos especially the Primer design, gene cloning videos. So thank you so much. Please do not stop making these videos. Would love to learn more about laboratory techniques, molecular and cell biology from your channel.

[hassanelsaid]

The best explanation I have came across so far

[muhammadwaqasluqman]

Wow. This is amazing Video. I have been looking for such videos for years. Uploaded in 2019, I wonder why it didn't show up in my search earlier. This is what I actually needed to see.
Thank you so much for this.

[dkumar]

Hey Jacob, Just awesome man...Just Awesome such a great video after a long time I watched!!

[akshu]

Your videos are a life saver. I am about to join a lab working on CRISPR CAS9 technique. I have done my PhD in Mechanobiology, an area very different from this. Your videos helped me to brush-up my Masters in Biotech knowledge. Thank you for the videos 🙏🏻

[mnzubyk]

Thank you so much for taking the time to do this. Wonderful video. Really quick, around 21:34, when you are pasting the 1500bp promoter region into Word, you pasted an extra "A" I believe before the beginning of the gene. There are two A's and I think there should be just one. When setting the EPD sequence retrieval tool from -1485 to 0, the 0 is the first base of the gene. So it doubles the last base, which is an "A". Awesome video though, really wonderful teaching.

[ashwinshukla]

very helpful video...keep uploading such contents.

[amandagong]

Hi, I found when we set "0 to TSS" in EPD, the last latter was the 0 position of the TSS, which is the first letter of TSS, here in your video was "A", so I think when we copy it, it should be deleted. :)

[dipanwitapaul]

Very much informative but easily explained video! thank you very much

[nilanjanaghosh]

Hi, first of all its a very helpful video. Could you please explain why did you consider only 1500 bp while adding the sequence range in Eukaryotic Promoter Database?

[Alasdair]

Very usuful and exhaustive for me. Thank you