🔴 Calculating Amount of Drug Needed for Cell Culture: Practical Guide

The typical workflow for cell culture (mammalian cell culture) involves thawing cells from liquid nitrogen and culturing in appropriate nutrient media. The nutrient media often has fetal bovine serum (aka fetal calf serum), penicillin, streptomycin and L-glutamine or Glutamax. In addition to these cell culture components, some additional components may be needed. for example, If the cell has been transduced and contains a transgene or if the cell requires a growth enhancer or additive. In this video we cover how to calculate the concentration of a required selection reagent.

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This video is about cell culture techniques, cell culture, cell culture basics, cell culture calculations and cell culture media preparations, Drug dosage calculation, Cell culture drug treatment, Drug concentration calculation, Drug preparation for cell culture, Drug dilution calculation, Pharmacology in cell culture, Drug dosing in vitro, Cell culture experimental design, drug treatment optimization, Lab math for cell culture, Pharmaceutical calculations, Cell culture protocols, Drug delivery in cell culture, Dosage calculation formula and Laboratory techniques in drug research.

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Why do we need to be able to do such calculations? In order to maximize all available storage space most solutions are stored in a concentrated form (known as stock). These solutions are then diluted to the required strength as and when required. This also means the same solution substance may be used for a different range of needed concentrations.