Introduction to single-cell RNA-Seq and Seurat | Bioinformatics for beginners

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This is was a quick introduction to single-cell RNA-sequencing technology. Watch out for more videos where I demonstrate how to process single-cell RNA-seq data using the #Seurat #R package.
I hope you liked the video. I look forward to your comments under the comments section!

Chapters:
0:00 Intro
0:48 scRNA-Seq vs bulk RNA-seq
1:50 Basic Terminologies
2:56 scRNA-seq Technologies
3:44 Packages for scRNAseq data
3:59 Understanding Seurat Object

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#bioinformagician #bioinformatics #genomics #beginners #tutorial #howto #omics #research #biology #ncbi #GEO #rnaseq #ngs #Seurat #R
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Single Cell Analysis has been a lonely world for me so far. Happy to find someone is putting up efforts to make it easier for starters!!

rohitsatyam
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Commendable. Thanks for uploading this useful video.

NishantSharma-fb
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I am a biologist learning scRNAseq from scratch. So I am glad to see your tutorial. Adding yours to the list of mentors! Please provide more details on how to analyze multiple 10x samples or replicates. The seurat vignette had the merge() function but I am not sure how to apply split.by comparing the features. Cheers for your channel!

kitdordkhar
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Thank you sister. I hope there will be more pratical projects soon

sakichan
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Can you make a video on pseudo time analysis from single cell data?

animatedbiologywitharpan
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Well what can I say, just perfect
Thank you :)

francis
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Request to review Giotto for spatial transcriptomic analysis. Your videos have been very helpful

michelleyibingwei
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Thank you for a helpful video! I could not find the video you mentioned in the basic terminologies part of this video. Would you please tell me where I can find it?

chrisdoan
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Mam can you please explain more about UMI and how to apply and tools

sheikhmujibur
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I was wondering how to make an assay within Seurat using metadata information. Would you please be able to share some pointers or a script I could repurpose? Thanks.

msumode
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Do you know about a tool for bulk rna seq in python? I am aware about scanpy for single cell rna seq but not for bulk. Very good video, thanks!

LanaDominkovic
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Dear Khushbu, your method of teaching is really superb. Guide me please in creating Seurat object of SCRNA Seq data of ArrayExpress like E-MTAB-7704. Thanking you in anticipation

hafeezuom
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Are you a Seurat advocate or package agnostic when it comes to single cell workflows in R? I'm just venturing into them now and this video is very helpful thanks.

stretch
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Hello Khushbu, I'm a beginner in R language and i want to create tSNE plot for single cell RNAseq data for embroids.
Like stages of embryos and my gene of interest
So I'm finding different to create dataset and idk what all information is required
Can you please help me asap ? Plzz

gunanadar
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Hi Khushbu, this video was really helpful and when I tried the integration tutorial, my R ran into vector exhaustion error and then the R session quit and my MacBook kinda crashed..so I wanted to ask you that which MacBook do you use ? Do you think it’s a RAM issue cuz I’m using 8gb RAM MacBook !

nandinipatel