Single-cell sequencing explained in 2 minutes

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What is single-cell sequencing? Why do single-cell sequencing? Single-cell sequencing is a complex process, but the fundamentals are relatively straight forward. Here I cover the basics which are needed before getting your feet wet in more complex single-cell topics.

Single-cells sequencing can be broken down into just a few basic steps:
1) Label an individual cell's RNA with unique barcodes
2) sequence the RNA from all cells and split the reads based on their attached barcode which are also sequenced
3) Downstream analysis...
  1. Sanbomics
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Комментарии
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Could you suggest me a simple reference for beginners regarding scRNA_Seq

kamarsedik
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Im not a scientist. I came here from cancer research. I gave it a thumbs up. Clear and informational.

cold_hardfacts
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Your videos are perfect. I appreciate the no-intro, straight to the meat style.

roshanpatel
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Great video and summation. Surprised it doesn't have more views!

michaelbailey
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Awesome! Very clear explanation, thank you <3

szymonjakubowski
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Thanks for the video! Could you maybe elaborate on common use cases for single cell sequencing? E.g. what questions could you answer after analysis? Just curious of the bigger picture where scs fits Thanks!

jappe
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Why is it easier to understand on what you are saying than to understand it on my language hahaha, ,

foweurn
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i dont get how the bead somehow tags each RNA transcript in the cell if it just sticks to the outside?

saveriov.p.
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Is UMI shows nFeature or nCount in the sc rna analysis?

neurostudywithme