Single Cell Sequencing - Eric Chow (UCSF)

Dr. Eric Chow gives an overview of single cell sequencing, explains why this approach is useful, and talks through the leading methods.

Single cell sequencing, as the name implies, allows researchers to examine the genomic information for individual cells. This provides an opportunity to examine cell-to-cell differences and identify cell subtypes, which provides insight into how specific cells function within and respond to their environment. Dr. Eric Chow begins his talk with an overview of single cell sequencing with a focus on RNA. He then goes on to outline the predominant approaches, including plate-based, microfluidic-based, and combinatorial indexing methods. He finishes by addressing approaches to single cell analysis that don’t rely on RNA, including methods that use DNA, proteins, and antibodies. He also reviews some of the benefits and limitations of analysis at the level of individual cells.

0:00 Start
0:31 Bulk vs. single cell analogy
4:58 Plate-based SMART-seq
7:08 DropSeq
13:54 Combinatorial Indexing
22:56 Conclusions

Speaker Biography:
Eric Chow is an assistant professor in the Department of Biochemistry and Biophysics and the Director of the Center for Advanced Technology (CAT) at the University of California, San Francisco. The CAT provides resources for UCSF labs wishing to use next generation sequencing techniques and Chow’s research program strives to develop new applications for NGS in pathogen diagnostics. Chow received his BA in molecular biology from the University of California, Berkeley and his PhD in biochemistry from UCSF.

Credits:
Karen Dell (iBiology): Producer
Eric Kornblum (iBiology): Videographer & Video Editor

[Keigan]

Please get Eric to do more of these videos, this is by far the best overview, and easiest to understand, that I’ve found. He’s so good at actually explaining in a way that is not overly technical on purpose. I feel that many in the field are gatekeeping their knowledge, instead of explaining in ways that others can understand.

[anastasiapivnyuk]

Really cool! Thank you Dr. Chow for this detailed and at the same time clearly structured talk!

[jey_jju]

Thank you so much for this introduction of sc-RNA Seq. This video helps me a lot to understand each steps even though I didn't have much of understanding about this field. Thank you again.

[BasicScienceSeries]

Thank you Dr. Eric for this wonderful talk. You have provided detailed information on Single Cell Sequencing and explained every detail of this technique. I found the lecture valuable and highly informative. Thank you very much iBiology Techniques for providing this valuable lecture.

[codewithluq]

This lecture is highly informative and greatly valuable. It has cleared most of my gray areas in the field of single cell analysis

[merrimac]

Thank you for the nice introduction of single cell sequencing methods!

[esanau]

17:13 quantifying proteins using DNA sequencing (CITE-Seq)
21:26 universal antibodies for a universal cell-surface protein that have unique barcodes for each cell to detect the unwanted doublets (droplet with one bead but two cells)

[meenukesarwani]

Dr Chow I love your videos. Please continue your good work. Its really thorough and well explained with theory behind each technique which are not explained from the product maker /companies.
Thank You!

[qutemango]

thank you for explaining these technologies so clearly!
it's very useful for me.

[catherinegrgicak]

Great presentation! Just great! I agree with all of the other positive comments. The content is great, easy to follow and Eric is a great presenter.

[anjadekanski]

These sequencing videos are really great! I hope you can also cover UMIs, spatial transcriptomics, and other such difficult concepts :)

[AliRazaShahSARSB]

Thank you, It was really good to understand the basics of single cell sequencing methods.

[marcuslcc]

Extemely helpful for newbies!!! Thank you Dr. Chow!!!

[karimali]

One of the best scSeq overviews out there. Keep it up you're doing great!

[jordanramilowski]

You look for something on YouTube. The video with Eric Chow pops up. You know its gonna be good...

[여가뮤직프로듀싱]

Thank you so much for this lovely talk. I think this is the best talk summarizing scRNAseq.

[samanwoymukherjee]

Excellent talk and lucid explanation of the biology Dr. Chow. Thanks iBiology, looking forward for such talks in future.

[vikas]

well mam from last few weeks, I am focusing on biochemistry portion, althrough I read all content of book but I'm not satisfied with that content, can you please answer or make vedio on my following queries:
(1) in lab whenever we isolate some compound and want know the structure of compound, how do we really formulate them, what are those techniques, are these techniques 100%accurate or just give approx idea
For example, I always wonder how scientist who has first isolated ATP molecule, how he give structure of ATP, infact how he isolate compound at level of molecule, (since i have isolate compound at level dna only) how cycle such as kreb cycle or glycolysis are performed in lab
Your entire vedio lecture have always motivated me, thanks a lot
I am from india

[valentynapolishchuk]

Great video, thank you! Very informative and easy to understand!

[beckysam]

Thank you for this coherent and excellent introduction!