Pyrosequencing

This video is about pyrosequencing method of DNA sequencing. Pyrosequencing is a sequencing technique in which, the incorporation of dNTP in the DNA is detected in The form of light. If dNTP is not incorporatred, no light is detected. hence the sequence information of the DNA can be easily obtained by detecting the light.

[kristenapostol]

OHMYGOSSH, this is PERFECT! Exactly what I needed for my exam tomorrow, and explained the purpose behind everything, too! Thank you so much!!!

[eneaventurini]

This video is really clear and precise. Thank you!

[rossmoorman]

Thank you. The PowerPoint the teacher gave for the test is garbage, but this clearly explains the concept.

[giddygiddybangbang]

What an amazing video! Thank you for the clear and concise explanation.

[studybooks]

thank youuuu!!!! I never understood how pyrosequencing diferentiate each nucleotide, until this video.

[솔방울-yr]

This video was so helpful for me to understand the principle of pyrosequencing. Thank you so much!

[manospapadopoulos]

Such a beautiful video. Made the concept crystal clear🙂

[noahjendrasheske]

Thanks! My professor did not say anything about the flow cell. This clears everything up.

[aliceyonemin]

That's so useful and easy to understand, thank you very much

[geovanniruiz]

Amazing explanation ! Congratulations and thank you!

[martapastori]

extremely useful!!! thank you very much :)

[mastersacademy]

This really amazing and very easy for learning.

[camilocastelblanco]

Bro, you make this topic very easy. Thank you

[replicationcellulaire]

There should be an issue here if I understand well. At 3', during the example, the added nucleotides are in the order : G A T C A G A T etc... However when the second template is "GC", dATP is added, and nothing happens for template 2, only template 1 gets light. But then there is dGTP then an other dATP, WHICH THIS TIME CAUSES LIGHT. Why does the first dATP did not cause light in template 2 ? Just a little error on the video or am I missing something ?
Great explanation otherwise

[priyakamboj]

This vedio make my concept clear thanku so much sir

[malt]

Bless you! You've just made my studying so much simpler! Thanks you!! :-D

[jozzatheg]

Fantastic vid! Thanks so much for creating this

[marianachartian]

Amazing video and great explanatn too but i juz have a silly doubt...can u plzz clarify..if we add a dNTP to the flow cell and LIGHT gets produced, then doesnt it mean dat the corresponding base in the template is complementary to the dNTP added?? For eg. if we add dGTP and get LIGHT, wont the corresponding base in the template be 'C' den?? The video shows dat it wud be 'G'....so im a bit confused..plz clarify sir..thank u in advance..😇

[rumad]

perfectly explained. thank you so much!

[FnaffFifii]

How do u know the specific sequence?
The dNTPs can be matching the nucleotides in the template strands randomly..