Pyrosequencing

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This gene sequencing lecture explains the mechanism of pyrosequencing. It also states the use od pyrosequencing in high through put DNA sequencing analysis in next generation sequencers.
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PPi is not going to bind with Ammonium persulfate (APS)
but is going to bind with adenosine 5´ phosphosulfate (APS)

SHAILENDRAKUMAR-zwpi

i don't think you have enough annotations

Jim-tytg

It's hard to watch this video with 15 subscribe annotations in front of it...

moemoney

the light intensity is proportional to the to the amount of incorporated nucleotides, not to the types of nucleotides

sebastianybarnegaraywende

Shomu ji your way of explaining is really good. but I want to make one important correction. you said Ammonium persulphate but it is actually adenosine 5´-phosphosulfate (APS) here, without this we wont get ATP. Right???

manishawalunj

Thank you so much... please keep going, we always need such an explicit lessons from you... may good bless you

fatimahaouam

this doesn't explain why different nucleotides make different intensity of light

tmk

simple and easy to understand, thanks

kirkferentzrocks

so this only allows to determine the map the order of base pairs in DNA strand?

roseb

The explanation is good, but in the end it got a bit too fast after luciferin is added, and the video suggestion at the top and the end card (to play the next video) is covering the slide.

RafTube

Is is an advert or an educational video? It is clearly u r doing this for money not to help. How are we suppose to see when u spam the screen with adverts and links.

khanyameyiswa