Label-free Live Cell Imaging: T-cells killing cancer cells - zoomed-in

In this movie (killing assay) you can see T-Cells and cancer cells.
The cancer cell line is MC38-OVA, a transduced colon cancer cell line that expresses the ovalbumin (OVA) model antigen.

T cells are coming from OT-I mice, those T-cells carry a transgenic T-cell receptor responsive to OVA residues 257-264 (SIINFEKL peptide) in the context of the MHC I H2kb.

In the killing assay, the same T-cells, which are now called “effectors” (thanks to the APC-mediated activation performed in the first experiment), are incubated with MC38-OVA cancer cells. Upon recognition of their target (the OVA residues on the MHC I H2kB of the cancer cells), T-cells induce the killing of the cancer cells. However, this does not happen all the time and many cancer cells survive.

Technology:
3D Cell Explorer microscope from Nanolive, Switzerland.
The 3D Cell Explorer is a high speed, high resolution and non-invasive live cell imaging microscope that can look deep inside biological systems. This allows you to record stunning 3D images of entire cells in just seconds and with a higher resolution than any conventional microscope on the market.

[nancyselzer]

I'm a retired microbiologist /immunologist. Your photography is remarkable. A+ and a big thumbs up.

[theangryjess]

Man. T cells don't get enough credit. They put in so much work to keep our bodies safe, respect man

[hikeclimbsail]

Thank you to all my cells working endlessly just to keep me healthy 🙏🏼🙏🏼🙏🏼

[justjustjustify]

There is a whole universe within us this is so miraculous

[timothysantama]

For you to live, there are warriors that guard you day and night. We should be respectful to our body mechanism

[markfsal]

Big respect to the men and women who do research and develop the drugs that help our immune system work. M

[techshabby]

Amazing. I've been sharing these videos with my bf because he's quick to take pills, and I'm trying to get him to realize your body is a powerhouse that's fighting for you every second and you have to give it the chance to to the work.

[todddixon]

Praise God for the workings of our bodies The Great Physician !

[battybethc]

The T-Cells are adorable! 😊❤️Such tiny warriors with all that power!👍😊 Bless them all! We all are worlds within the world! Amazing! 😊👍

[clespo]

igrotum is not just a treatment; its a breakthrough in cancer care. I highly recommend it to anyone on the cancer journey.

[KnowledgeSponge]

Man I could watch this all day! Gives a new perspective on a healthy body!!

[virginiekutiaka]

I thank God for being marvellous and protective. Only you yhe the creator could do such wonder. Forever grateful 🙏

[Rahel]

This comment will help you

When you are stressed or in depression, your T cells are weakened, that's why they can't kill the cancer in your body and the cancer is growing bigger and bigger.

How possible you are expecting your troops, the T-cells, in your body to be strong to kill the cancer if you yourself are letting yourself to fall in depression?

But if you are happy, your t cells are very strong and they will easily kill any harm beings in your body such as cancer cells.

The point is be happy, don't let stress control you, just let it go

[maineeveryday]

Why does this make me cry in amazement

[terryglendening]

Next time I start a sports team, it won't be the "Wildcats" or the "Daredevils", it will be the "T-cells"!!

[billb]

To strengthen Tcells take 55mcg selenium, 5000 D, protein, I recommend egg or whey. Be blessed ❤

[NK-xwok]

Wow! The little cells appear to ne filed with electricity !
little lightning connections pulsating about, like a little brain!
It’s amazing!

[Jakoclubs]

Just finished a immunology class, this is over-the-top cool! Thank you!

[javierkevinfranco]

These videos are astonishing, even though I have zero idea what's going on.

[redeagle]

Wow! Thanks for showing how our body protects.😊