Label-free Live Cell Imaging: T-cells killing colon cancer cells

In this video capture (killing assay) you can see T-Cells and cancer cells.

The cancer cell line is MC38-OVA, a transduced colon cancer cell line that expresses the ovalbumin (OVA) model antigen.

T cells are coming from OT-I mice, those T-cells carry a transgenic T-cell receptor responsive to OVA residues 257-264 (SIINFEKL peptide) in the context of the MHC I H2kb.

In the killing assay, the same T-cells, which are now called “effectors” (thanks to the APC-mediated activation performed in the first experiment), are incubated with MC38-OVA cancer cells. Upon recognition of their target (the OVA residues on the MHC I H2kB of the cancer cells), T-cells induce the killing of the cancer cells. However, this does not happen all the time and many cancer cells survive.

Technology:

3D Cell Explorer microscope from Nanolive, Switzerland.

The 3D Cell Explorer is a high speed, high resolution and non-invasive live cell imaging microscope that can look deep inside biological systems. This allows you to record stunning 3D images of entire cells in just seconds and with a higher resolution than any conventional microscope on the market.

[michaeljanes]

The marvel of the human T- cell. A real miracle of God's design.

[lamckinney]

Amazing! Thanks for sharing your knowledge. !!!😊

[okranendonk]

Fantastic de minunat este creatia Lui Dumnezeu!El da incercarea dar si infaptuirea❤

[PollyannaLeos]

WOW 😮! Wish this was around when my father died of asbestos cancer 😢!

[robertjones]

This is in controlled laboratory conditions.There are some possibilities with this but we are years away.

[uwadile]

Doctors pls look for the mendication for this mad sickness

[ignaciacabrera-ekzt]

Que increíble es nuestro cuerpo..somos como un universo de vida nanoscopica❤

[sandradalessandro]

They offer. CAR T. CELL THERAPY. At many cancer centers. Now!! I did mine at FOX CHASE. In PHILLY

[de-janeniles]

Science test need to humble themselves before God the Almighty. He who created ALL things, and for His pleasure, they were created; you are worthy, oh Lord!

[MA-]

This is cool, but how was this captured/recorded?

[salvatoregiacomuzzi]

If you watch this how can we believe that every cell is equivalent like another. It seems that every cell has its own characteristic 😢😢😢😢😢😢

[koetje]

MORE OF THESE VIDEOS AND SHORTS MORE MORE MORE MORE MORE MORE

[NMSRF]

One more explanation that our bodies were ment to live for ever ❤ But death came through sin..😔 God did create perfection at the beginning tho❤