Macrophages imaging in a live human ovarian cancer tumour slice

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Time-lapse experiment of a live ex vivo human High Grade Serous Ovarian Cancer omentum metastatic tumour slice, stained with CD11b (red), EpCAM (green) and fibronectin (pink).

Images are maximum intensity projections of 8 z slices, acquired every 30 seconds for 30 minutes.

The fibronectin staining shows the extracellular matrix, CD11b shows tumour-associated macrophages (TAMs) and EpCAM shows tumour cells.

This video highlights TAMs' movements within a live human tumour microenvironment.
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