How to Make Agar Plates for Growing Mushrooms

In this video I show you my process for making sterilized nutrient rich agar plates for growing mushroom cultures.

Agar Recipe for Malt Extract Agar:

For every 1000 ml water add:

20 g Agar Powder
20 g Barley Malt Extract
2 g Nutritional Yeast

Sterilize for 45 minutes at 15 PSI. Pour into plate in a sterile environment.

[vermeer]

Best editing and fastest presentation of information I've ever seen on YouTube. You are a true artist, Tony.

"Trifles make perfection, and perfection is no trifle. Genius is eternal patience." - Michelangelo Buonarroti

[jeremywilliamson]

dude, where have you been all my life? i've been learning mycology for a while now and had so many simple yet significant questions that all other online sources have failed to answer until now. thank you and keep up the good work! i'm so happy to find someone willing and capable to include the attention to detail that is so necessary to succeeding in this field. i will be recommending your videos to all my noob myco peers, and hopefully before too long i will have developed the skills and confidence to share my own wisdom via quality video tutorials like these. respect :)

[MsK-xmvw]

You are so wonderfully informative. By teaching the ignorant like myself, you are changing lives. It takes a community to save the world, a great start.

[senkodan]

I like how the dog looks through the window. Very calm.

[tanuambia]

I have an exam tomorrow on Mushroom cultivation technology and I can't explain how helpful your videos are proving to me. Your explanations and physical demonstration are so easy to understand. My book is really annoying and I was having a hard time preparing for tomorrow so thank you for such amazing content. And writing this currently, I have already watched 10 of your videos.

[ghost-carnage]

I used a pressure cooker for the first time today. I also made agar dishes and innoculated them for thr first time. First grow ever actually, hoping it goes well but if not thats why i chose to learn the agar! Thanks !

[davecoolen]

Great video, very informative, quick and to the point. No wasted banter and you covered everything! Thanks

[TripleDDDD]

Great Video! As a passionate Home Brewer I can tell you that you‘ll find all the materials in good brew stores. Including petri dishes Agar agar and off course the rye which is used in the mason jars. These materials are also used for yeast cultivation.

[PRWelke]

A whiskey bottle? Tell me you're from Alberta without telling me you're from Alberta...

Love it!

[judithlee]

Thank you for the great information! I've been using your agar recipe to grow oyster mushrooms and have had very good results. Also, I love your dog in the background...very stoic!!

[Jayaditya_shrivastava]

Tony is the best guy, when it comes to disseminating Mycology Knowledge! ❤️

[DarthSinistris]

Parafilm is the coolest material on earth. I used to LOVE playing with that shit when I used to work at a microbiology lab.

[mattnsim]

Your videos are the best on youtube for mushroom growing. Im sorry to point out that u didnt mention putting water in the pressure cooker. Ive never used a pressure cooker and almost started mine without water. I went back to the part in the video where you put the bottle in the pressure cooker and saw water in the bottom. I love your videos and pray for more content from you, im just worried that a newby may not add water to his cooker... love your work.

[chloemypet]

I have learned so much from you! Please don't stop teaching!

[pnwwildadventures]

Accidentally had my playback at 1.5 he was very up beat

[freemab]

Excellent video.
I recommend using a canning jar, not a whiskey bottle. If you must use a bottle, choose one that has rounded corners at the bottom. If it has an abrupt transition from the walls to the bottom, it is much more likely that the bottom will break off the bottle when heating or cooling. An abrupt change in the thickness of the glass could also be a problem. The ideal container for heating or chilling is either borosilicate glass or spherical in shape or both. (Chemists use round-bottomed flasks.) The closer your bottle is to this ideal, the less likely you'll lose your bottle and contents in the pressure canner.
BTW, pressure canners are moderately common at garage sales and flea markets. I've bought two this was and passed up many more. Not all of them are as large as Tony's seems to be, but I've never seen one that couldn't handle seven 1-quart jars.

[cbaymac]

Great video Tony. Very helpful and professional. We really enjoy your Lions Mane and Cordycepts supplement powers.

[kiya]

TaThank you from thousands of miles away for the tutorials

[krainey]

Great job on the clip..
Very informative and clearly presented I will be trying soon. I need to make a flow hood .

[shrikantsatale]

Short sweet and best information !! Thanks

Few questions
1) After inoculations, how to incubate these agar plates? Do this really require BOD incubator? Or any cost saving ideas?

2) How many times can we subculture these primary culture? Does passaging multiple times affect productivity?

Looking forward for your experienced guidance