Plate Pouring Protocol

For the full written Plate Pouring protocol, visit:
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0:00 Intro
0:36 Materials
2:02 Preparing Agar Mix
2:53 Autoclave
3:34 Bench Setup
4:42 Set the Water Bench
6:04 Pouring
7:19 Solidification and Storage
7:48 Testing
8:26 Review

[flyingmorningdew]

this is an amazing video perfectly made, major kudos to Addgene for sharing the science!

[vikramjitsingh]

very nicely explained and the comic illustration lightens the mood as compared to some dead serious science type labs out

[Bing]

thank you so much for this wonderful video ill make sure to subscribe to your channel

[andrerodon]

Nice video, but the high pressure in the autoclave is not to keep the solution from boiling over - it raises the temperature at which water boils, thereby allowing for sterilization of the solution/substrate by killing microbes that can survive the normal boiling point of water.

[HuyenPhan-irwo]

This was in my homework assignment as an OS.

[udayawimalasiri]

Informative and interesting. Thanks for sharing.

[christinerodriguez-rutkows]

What do you use for absorbent material?

[naveen]

How do you dry them overnight at room temp. after adding antibiotic? antibiotic half life is 12-14 hrs, right? I think this procedure is for plates without antibiotic. Or else, we can immediately store the plates after solidification at 4C if the antibiotic is added. In this procedure, the colonies are supposed to be grown in -ve plate too because the antibiotic has already been degraded.

[christinerodriguez-rutkows]

Is there a way to cool plates faster, such as in a 4'C fridge? Is there a downside for cooling them too quickly?

[skaweber]

What is the exact type of pipet are you using to pipet the liquid agar onto the petri dishes?

[tartanhandbag]

if your agar goes solid, you should preferably NOT autoclave it again as this will degrade the nutrients eg. caramalising sugars, which can have metabolic consequences. this of course is dependant on what you're doing with the bacteria and so is permissable in certain scenarios.

[geinstein]

I do think it would be better practice to pour plates in front of your flow hood instead

[bkerbal]

can i make plates at home without being able to steralise things. my partner is interested in this field as a hobby (i know nothing about it) and wanted to get the stuff to make plates at home but not sure if there is a point if we dont have an autoclave. pls someone let me know asap if its possible to do at home so i know if i should order the powders and dishes

[muslimlegends]

How much Agar conc should i use for one petri plate?

[dannydelao]

Does the flame act as a laminar flow hood as in it prevents contaminants from falling on the plates ?

[ruqaraghuli]

Can you please show us a video of different contaminated C-elegans plates? And the causes for such contamination???

[dp_edc]

What kind of bottles are those that you use for pours, looking for something easier to pour out of than the mason jars i am currently using, one screw up and it pour down the side rendering the rest of the batch useless since it has then came in contant with the outer jar meaning its no longer sterile.

[dylanbaker]

Why would you not spread both nonresistant and resistant bacteria on the same plate? For example half and half

[joekub]

great job telling inexperienced you tubers to light their burner before spraying alcohol.

[mr.buttercutter]

Sir please explain the importance of control plate.im still waiting for your reply