How to select the column dimension of an HPLC column?

#hplc #column #pharma #interview #methoddevelopment

🖥️Course offered by Pharma Growth Hub🖥️

Many pharma professionals have chosen Pharma Growth Hub as their career acceleration partner, now it’s your turn!

27 courses worth Rs. 1,16,690/- are available for just *Rs. 6990/-* This offer may not stay for long, so take action.

Please find the main module details below...

💎1. Assay method validation mastery 💎
» Everything you need to know to perform assay validation
» Preparation of conc. matrix, protocol, calculations, report wrt global requirements

💎2. Dissolution method validation mastery 💎
» Everything you need to know to perform dissolution validation
» Preparation of conc. matrix, protocol, calculations, report wrt global requirements

💎3. Related substances method validation mastery 💎
» Everything you need to know to perform RS validation
» Preparation of conc. matrix, protocol, calculations, report wrt global requirements

💎4. Residual Solvents method validation mastery 💎
» Everything you need to know to perform Res sol. validation
» Preparation of conc. matrix, protocol, calculations, report wrt global requirements

💎5. Extractables & Leachables Blueprint 💎
» What is E&L
» What is the source of E&L
» How to use Risk-based approach for safety assessment
» What are Thresholds for E&L
» How to design E&L Study

💎6. Stability Study Mastery 💎
» Basic terms in stability study
» Stability study for submission
» Preparation of stability protocol, report
» IQ, OQ,PQ of stability chamber

💎7. Method development blueprint 💎
» HPLC, GC, Dissolution, Spectroscopy Method Development

💎8. Cleaning validation blueprint 💎
» How to perform cleaning validation

💎9. Mutagenic & Nitrosamines 💎
» Understand regulatory aspects of mutagenic & nitrosamines
» Testing need of Mutagenic & Nitrosamine into your product
» LOQ requirement for Nitrosamine test procedure
» Limit for new Nitrosamine impurity
» Limit for mutagenic impurity

💎10. QMS Mastery 💎
» Incident, OOS, OOT, CAPA, Change control
» QMS examples

💎11. ICH Quality Blueprint 💎
» Comprehensive course on ICH Quality guidelines

💎12. FDA 483 Observations 💎
» Comprehensive info on FDA 483 recd. By Indian Pharma (10-12 yrs)

💎13. Impurity Management blueprint 💎
» Limit for known, unknown, total impurity
» Limit for residual solvents
» Release & Stability specification for Impurity

💎14. Instrument Calibration Mastery 💎
» Calibration of common lab instrument
» Calibration templates & formats

💎15. Instrument Qualification mastery 💎
» Qualification of common lab instruments
» Calibration templates & formats

💎16. Information Technology Blueprint 💎
» Electronic data management
» Server Data Backup, Retrieval, and Verification
» Preparation, Execution & Review of CSV

💎17. Specification design blueprint 💎
» Specification for API/Finished product
» Specification for unknown impurity
» Specification for known impurity

💎18. Pharma tech blueprint 💎
» More than 90+ videos on various topics of QC, QA, AR&D

💎19. Concentration matrix design mastery 💎
» Concentration matrix for method validation
» Confirmation on selected weighing and dilution details
» Mapping expected concentrations for API/Impurities

💎20. Calculation formula design mastery 💎
» Reporting and rounding of analytical results
» Basic calculation functions in excel
» Basic calculation formulas in Analytical chemistry
» Preparation of automated calculation sheet

💎21. Concentration design blueprint 💎
» Conc. Optimization for assay, RS, Dissolution, Residual solvent

💎22. Lab Operation Blueprint 💎
» Basic Lab Set-Up Guidance

💎23. Interview Question Model 💎
» Likely Interview Questions

💎24. Job Search Strategy 💎
» 3 Month job search plan
» Job search tracker
» How to connect with TAMs
» How to communicate with TAM

💎25. Resume & LinkedIn Blueprint 💎
» Resume optimization
» LinkedIn optimization
» Resume writing
» Converting resume templates

💎26. Job interview blueprint 💎
» Job interview checklist
» Job INTERVIEW questions
» Phrases to avoid
» Power of words

💎27. Inner circle calls 💎
» Previously recorded sessions
» Opportunity to network with PGH Stars

Alternate payment options(GPay/PhonePe): 9766383968
WhatsApp @9766383968 for more info…

[pankajnagwani]

Sir please make a short video on method of preparation of buffer of different pH with calculation

[rajaryanbiotech]

Regarding poresize, you mentioned 1000A to 4000A for higher molecular weight of protein and vaccine, Need to understand what is the molecular weight of protein and vaccine? Also, please mention this is for purification related, ie downstream process. For Analytical estimation purpose for higher molecular weight, example upto 150 KD we can use 300A for LC.

[arafatjakir]

Sir, i am from bangladesh. I like your video. I want a video related to elemental impurities.

[dils]

Nice sir,

wider pore size columns having Higher surface area or lower??

[chandbhaiinamdar]

Good morning sir

Which column is suitable for surfactant molecule. I am getting four peak on chromatography.